{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:50:19Z","timestamp":1790740219812,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,6,6]],"date-time":"2011-06-06T00:00:00Z","timestamp":1307318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p><jats:italic>Rego EB, Inubushi T, Miyauchi M, Kawazoe A, Tanaka E, Takata T, Tanne K. Ultrasound stimulation attenuates root resorption of rat replanted molars and impairs tumor necrosis factor\u2010\u03b1 signaling<\/jats:italic> in vitro<jats:italic>. J Periodont Res 2011; 46: 648\u2013654. \u00a9 2011 John Wiley &amp; Sons A\/S.<\/jats:italic><\/jats:p><jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> A therapeutic protocol to minimize root resorption induced by tooth replantation has not yet been universally established. In this context, noninvasive modality such as ultrasound therapy have been a focus of increased interest. This study aimed to evaluate the inhibitory effect of ultrasound therapy on root resorption of replanted rat molars. In addition, the study aimed to promote insights into the mechanism through which ultrasound mediates the metabolism of periodontal cells <jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> An experimental model of tooth replantation in rats, involving luxation and immediate replacement of the maxillary first molars, was used to assess the inhibitory effect of an ultrasound\u2010therapy regimen (15\u2003min of exposure to ultrasound, each day for 21 d) on root resorption. Moreover, the effect of ultrasound on osteoclastogenesis\/cementoclastogenesis was examined <jats:italic>in vitro<\/jats:italic> using a mouse osteoblastic stromal cell line (ST2) and a mouse cementoblastic cell line (OCCM\u201030).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The area of root resorption lacunae was statistically decreased (<jats:italic>p<\/jats:italic>\u2003&lt;\u20030.01) in the ultrasound\u2010treated sample. In addition, immunohistochemical staining, using murine TNF\u2010\u03b1 polyclonal antibody, failed to detect tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) protein in the ultrasound\u2010treated sample compared with the control. An <jats:italic>in vitro<\/jats:italic> study showed that the lipopolysaccharide (LPS)\u2010induced expression of <jats:italic>Tnfalpha<\/jats:italic> mRNA was significantly reduced by ultrasound therapy in both osteoblastic and cementoblastic cells. Moreover, the TNF\u2010\u03b1\u2010induced up\u2010regulation of <jats:italic>Rankl<\/jats:italic> mRNA was also inhibited by ultrasound.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Ultrasound may contribute to the reduction of the trauma\u2010induced inflammatory reaction through impairment of the TNF\u2010\u03b1 signaling pathway. It is therefore suggested that ultrasound shows potential as a therapeutic tool to optimize the regenerative potential of periodontal tissues on replanted teeth.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2011.01384.x","type":"journal-article","created":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T03:28:48Z","timestamp":1307417328000},"page":"648-654","source":"Crossref","is-referenced-by-count":19,"title":["Ultrasound stimulation attenuates root resorption of rat replanted molars and impairs tumor necrosis factor\u2010\u03b1 signaling <i>in vitro<\/i>"],"prefix":"10.1111","volume":"46","author":[{"given":"E. 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