{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:57:17Z","timestamp":1791212237540,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,8,17]],"date-time":"2011-08-17T00:00:00Z","timestamp":1313539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p><jats:italic>Trindade SC, Olczak T, Gomes\u2010Filho IS, Moura\u2010Costa LF, Cerqueira EMM, Galdino\u2010Neto M, Alves H, Carvalho\u2010Filho PC, Xavier MT, Meyer R. Induction of interleukin (IL)\u20101\u03b2, IL\u201010, IL\u20108 and immunoglobulin G by Porphyromonas\u00a0gingivalis HmuY in humans. J Periodont Res 2012; 47: 27\u201332. \u00a9 2011 John Wiley &amp; Sons A\/S<\/jats:italic><\/jats:p><jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold>  <jats:italic>Porphyromonas gingivalis<\/jats:italic>, an anaerobic gram\u2010negative bacterium, is associated with chronic periodontitis. This study was undertaken to evaluate the production of interleukin (IL)\u20101\u03b2, IL\u20108 and IL\u201010 by human peripheral blood mononuclear cells (PBMC) stimulated with <jats:italic>P.\u00a0gingivalis<\/jats:italic> antigens and to assess the levels of serum immunoglobulin (Ig)G, IgA and IgG subclasses raised against <jats:italic>P.\u00a0gingivalis<\/jats:italic> HmuY protein.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> PBMC from patients with chronic periodontitis (CP) and from nonperiodontitis (NP) control subjects were stimulated with <jats:italic>P.\u00a0gingivalis<\/jats:italic> antigens, and the cytokine levels in the culture supernatants were determined by ELISA. The specificity of serum antibodies raised against HmuY was analyzed by Western blotting and by ELISA.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Compared with the NP controls, the CP patients produced higher levels of total serum IgG and IgG1 specific for <jats:italic>P.\u00a0gingivalis<\/jats:italic> HmuY. No differences were found between CP and NP groups in the production of IL\u20101\u03b2 and IL\u20108 by PBMC stimulated with total <jats:italic>P.\u00a0gingivalis<\/jats:italic> antigens. Only <jats:italic>P.\u00a0gingivalis<\/jats:italic> lipopolysaccharide (LPS) induced higher levels of IL\u201010 in the CP group. Higher levels of IL\u20101\u03b2 and IL\u201010 were induced by HmuY than by other antigens derived from the wild\u2010type <jats:italic>P.\u00a0gingivalis<\/jats:italic> strains. In contrast, total antigens derived from the <jats:italic>hmuY<\/jats:italic>\u2010deletion mutant strain induced the production of significantly higher levels of IL\u20108 and significantly lower levels of IL\u20101\u03b2.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our data suggest that <jats:italic>P.\u00a0gingivalis<\/jats:italic> HmuY may be considered an immunogenic protein associated with host\u2013pathogen interactions.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2011.01401.x","type":"journal-article","created":{"date-parts":[[2011,8,18]],"date-time":"2011-08-18T04:18:53Z","timestamp":1313641133000},"page":"27-32","source":"Crossref","is-referenced-by-count":30,"title":["Induction of interleukin (IL)\u20101\u03b2, IL\u201010, IL\u20108 and immunoglobulin G by <i>Porphyromonas\u00a0gingivalis<\/i> HmuY in humans"],"prefix":"10.1111","volume":"47","author":[{"given":"S. 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