{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T03:14:40Z","timestamp":1789528480137,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,9,12]],"date-time":"2011-09-12T00:00:00Z","timestamp":1315785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p><jats:italic>Sugita N, Iwanaga R, Kobayashi T, Yoshie H. Association of the Fc\u03b3RIIB\u2010nt645+25A\/G polymorphism with the expression level of the Fc\u03b3RIIb receptor, the antibody response to<\/jats:italic> Porphyromonas gingivalis <jats:italic>and the severity of periodontitis. J Periodont Res 2012; 47: 105\u2013113. \u00a9 2011 John Wiley &amp; Sons A\/S<\/jats:italic><\/jats:p><jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Human Fc\u03b3RIIb is an immunoglobulin G (IgG) receptor that inhibits the activation of B lymphocytes through cross\u2010linking with the B\u2010cell receptor via immune complexes. This function acts as a negative regulator of antibody production. Our previous studies have demonstrated the gene polymorphisms in Fc\u03b3RIIb to be associated with periodontitis. In this study, we presented a polymorphism \u2013<jats:italic>Fc\u03b3RIIB<\/jats:italic>\u2010nt645+25A\/G (rs2125685) \u2013 in intron 4 and analyzed its functional relevance to periodontitis. We examined whether the <jats:italic>Fc\u03b3RIIB<\/jats:italic>\u2010nt645+25A\/G polymorphism is associated with periodontal parameters, the IgG response to the periodontopathic bacterium <jats:italic>Porphyromonas gingivalis<\/jats:italic> and\/or the expression level of Fc\u03b3RIIb on peripheral B lymphocytes.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Thirty\u2010two patients with chronic periodontitis were genotyped with nested PCR and by direct sequencing of genome DNA. The levels of serum IgG and of specific IgG subclasses for <jats:italic>P.\u00a0gingivalis<\/jats:italic> sonicate and for the recombinant 40\u2010kDa outer membrane protein (OMP) were determined. The expression levels of Fc\u03b3RIIb on peripheral B lymphocytes from 19 healthy donors were measured by flow cytometry.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Patients with the <jats:italic>Fc\u03b3RIIB<\/jats:italic>\u2010nt645+25AA genotype showed significantly higher mean clinical attachment levels compared to patients with the Fc\u03b3<jats:italic>RIIB<\/jats:italic>\u2010nt645+25GG genotype (<jats:italic>p\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.003) and a significantly lower IgG response to <jats:italic>P.\u00a0gingivalis<\/jats:italic> sonicate and to the 40\u2010kDa OMP. The expression levels of Fc\u03b3RIIb protein on the cell surface in peripheral B lymphocytes were higher in healthy donors with the <jats:italic>Fc\u03b3RIIB<\/jats:italic>\u2010nt645+25AA genotype than in those with the <jats:italic>Fc\u03b3RIIB<\/jats:italic>\u2010nt645+25GG genotype (<jats:italic>p\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.03).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The higher expression levels of Fc\u03b3RIIb in subjects with the <jats:italic>Fc\u03b3RIIB<\/jats:italic>\u2010nt645+25AA genotype may induce a lower level of production of IgG against <jats:italic>P.\u00a0gingivalis<\/jats:italic> and therefore more severe periodontitis.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2011.01411.x","type":"journal-article","created":{"date-parts":[[2011,9,12]],"date-time":"2011-09-12T12:10:10Z","timestamp":1315829410000},"page":"105-113","source":"Crossref","is-referenced-by-count":6,"title":["Association of the Fc\u03b3RIIB\u2010nt645+25A\/G polymorphism with the expression level of the <i>Fc\u03b3<\/i>RIIb receptor, the antibody response to <i>Porphyromonas gingivalis<\/i> and the severity of 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