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J Periodont Res 2012; 47: 532\u2013542. \u00a9 2012 John Wiley &amp; Sons A\/S<\/jats:italic><\/jats:p><jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Nonsurgical periodontal treatment controls periodontal inflammation. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are implicated both in the destruction and in the healing of periodontal tissues. The aim of the present study was to compare the mRNA expression of <jats:italic>MMP\u20101<\/jats:italic>, <jats:italic>\u20103<\/jats:italic>, <jats:italic>\u20108<\/jats:italic>, <jats:italic>\u20109<\/jats:italic> and <jats:italic>\u201013<\/jats:italic> and <jats:italic>TIMP\u20101<\/jats:italic> in chronic periodontitis before and after initial periodontal treatment.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Ninety gingival samples were harvested from 30 patients with chronic periodontitis (15 nonsmokers and 15 smokers) before and after nonsurgical treatment and from 30 periodontally healthy control subjects (15 nonsmokers and 15 smokers). Clinical parameters were assessed before and after treatment. Total RNA was isolated, and mRNA expression of <jats:italic>MMPs<\/jats:italic> and <jats:italic>TIMP\u20101<\/jats:italic> was assessed by RT\u2010PCR.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Periodontal treatment significantly increased <jats:italic>TIMP\u20101<\/jats:italic> expression and decreased the ratios of <jats:italic>MMPs<\/jats:italic>\/<jats:italic>TIMP\u20101<\/jats:italic>. Post\u2010treatment, nonsmokers with periodontitis had significantly higher <jats:italic>MMP\u20108<\/jats:italic> and <jats:italic>TIMP\u20101<\/jats:italic> expression than healthy nonsmokers, and smokers with periodontitis had significantly higher <jats:italic>MMP\u201013<\/jats:italic> and <jats:italic>TIMP\u20101<\/jats:italic> expressions than healthy smokers. Post\u2010treatment, smokers had significantly higher <jats:italic>TIMP\u20101<\/jats:italic> expression and lower <jats:italic>MMP\u20108<\/jats:italic>\/<jats:italic>TIMP\u20101<\/jats:italic> ratio than nonsmokers. Post\u2010treatment, there was no correlation among <jats:italic>MMPs<\/jats:italic>, and the expression of <jats:italic>MMPs<\/jats:italic> and <jats:italic>TIMP\u20101<\/jats:italic> was not correlated with clinical measurements.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Periodontal treatment increased <jats:italic>TIMP\u20101<\/jats:italic> expression and decreased the ratios of <jats:italic>MMPs<\/jats:italic>\/<jats:italic>TIMP\u20101<\/jats:italic> in chronic periodontitis. The post\u2010treatment increase in <jats:italic>TIMP\u20101<\/jats:italic> expression was higher for smokers. The <jats:italic>TIMP\u20101<\/jats:italic> expression was higher post\u2010treatment than in health. Post\u2010treatment, <jats:italic>MMP\u20108<\/jats:italic> expression was higher in nonsmokers with periodontitis than in healthy nonsmokers, whereas <jats:italic>MMP\u201013<\/jats:italic> expression was higher in smokers with periodontitis than in healthy smokers.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2011.01465.x","type":"journal-article","created":{"date-parts":[[2012,4,4]],"date-time":"2012-04-04T07:50:33Z","timestamp":1333525833000},"page":"532-542","source":"Crossref","is-referenced-by-count":18,"title":["Expression of <i>MMPs<\/i> and <i>TIMP\u20101<\/i> in smoker and nonsmoker chronic periodontitis patients before and after periodontal 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