{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T15:41:38Z","timestamp":1784302898212,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,2,20]],"date-time":"2012-02-20T00:00:00Z","timestamp":1329696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p><jats:italic>Iwayama T, Yanagita M, Mori K, Sawada K, Ozasa M, Kubota M, Miki K, Kojima Y, Takedachi M, Kitamura M, Shimabukuro Y, Hashikawa T, Murakami S. Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells. J Periodont Res 2012; 47: 563\u2013571. \u00a9 2012 John Wiley &amp; Sons A\/S<\/jats:italic><\/jats:p><jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Adiponectin is a cytokine constitutively produced by adipocytes and exhibits multiple biological functions by targeting various cell types. However, the effects of adiponectin on primary gingival fibroblasts and periodontal ligament cells are still unexplored. Therefore, we investigated the effects of adiponectin on gingival fibroblasts and periodontal ligament cells.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> The expression of adiponectin receptors (AdipoR1 and AdipoR2) on human gingival fibroblasts (HGFs), mouse gingival fibroblasts (MGFs) and human periodontal ligament (HPDL) cells was examined using RT\u2010PCR and western blotting. HGFs and MGFs were stimulated with interleukin (IL)\u20101\u03b2 in the presence or absence of adiponectin, and the expression of IL\u20106 and IL\u20108 at both mRNA and protein levels was measured by real\u2010time PCR and ELISA, respectively. Furthermore, small interfering RNAs (siRNAs) in MGFs were used to knock down the expression of mouse AdipoR1 and AdipoR2. The effects of adiponectin on the expression of alkaline phosphatase (<jats:italic>ALP<\/jats:italic>) and runt\u2010related transcription factor 2 (<jats:italic>Runx2<\/jats:italic>) genes were evaluated by real\u2010time PCR. Mineralized nodule formation of adiponectin\u2010treated HPDL cells was revealed by Alizarin Red staining.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> AdipoR1 and AdipoR2 were expressed constitutively in HGFs, MGFs and HPDL cells. Adiponectin decreased the expression of IL\u20106 and IL\u20108 in IL\u20101\u03b2\u2010stimulated HGFs and MGFs. AdipoR1 siRNA in MGFs revealed that the effect of adiponectin on reduction of IL\u20106 expression was potentially mediated via AdipoR1. Adiponectin\u2010treated HPDL cells promoted the expression of <jats:italic>ALP<\/jats:italic> and <jats:italic>Runx2<\/jats:italic> mRNAs and up\u2010regulated ALP activity. Furthermore, adiponectin enhanced mineralized nodule formation of HPDL cells.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our observations demonstrate that adiponectin exerts anti\u2010inflammatory effects on HGFs and MGFs, and promotes the activities of osteoblastogenesis of HPDL cells. We conclude that adiponectin has potent beneficial functions to maintain the homeostasis of periodontal health, improve periodontal lesions, and contribute to wound healing and tissue regeneration.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2012.01467.x","type":"journal-article","created":{"date-parts":[[2012,2,20]],"date-time":"2012-02-20T07:58:58Z","timestamp":1329724738000},"page":"563-571","source":"Crossref","is-referenced-by-count":29,"title":["Adiponectin regulates functions of gingival fibroblasts and periodontal ligament 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