{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:33:57Z","timestamp":1790026437943,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,26]],"date-time":"2012-03-26T00:00:00Z","timestamp":1332720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p><jats:italic>Xie H, Hong J, Sharma A, Wang B\u2010Y.<\/jats:italic> Streptococcus cristatus <jats:italic>ArcA interferes with<\/jats:italic> Porphyromonas gingivalis <jats:italic>pathogenicity in mice. J Periodont Res 2012; 47: 578\u2013583. \u00a9 2012 John Wiley &amp; Sons A\/S<\/jats:italic><\/jats:p><jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold>  <jats:italic>Porphyromonas gingivalis<\/jats:italic> has been implicated as one of the major pathogens in chronic periodontitis, an infectious disease affecting the majority of the adult population. We have previously demonstrated that a surface protein, arginine deiminase (ArcA), of <jats:italic>Streptococcus cristatus<\/jats:italic> represses production of <jats:italic>P.\u00a0gingivalis<\/jats:italic> long fimbriae and interrupts the formation of <jats:italic>P.\u00a0gingivalis<\/jats:italic> biofilms <jats:italic>in vitro<\/jats:italic>. Our <jats:italic>in vivo<\/jats:italic> studies have also shown that the distribution of <jats:italic>P.\u00a0gingivalis<\/jats:italic> and <jats:italic>S.\u00a0cristatus<\/jats:italic> in human subgingival plaque is negatively correlated. The objective of this study was to determine if <jats:italic>S.\u00a0cristatus<\/jats:italic> ArcA inhibits <jats:italic>P.\u00a0gingivalis<\/jats:italic> colonization and attenuates its subsequent pathogenesis in alveolar bone loss in the murine oral cavity.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> A wild\u2010type strain of <jats:italic>S.\u00a0cristatus<\/jats:italic> (CC5A) and its <jats:italic>arcA<\/jats:italic> knockout mutant (ArcAE) were used as initial colonizers in the oral cavity of BALB\/cByJ mice. Colonization of <jats:italic>P.\u00a0gingivalis<\/jats:italic> on the existing <jats:italic>S.\u00a0cristatus<\/jats:italic> biofilms was assessed by quantitative PCR, and <jats:italic>P.\u00a0gingivalis<\/jats:italic>\u2010induced alveolar bone loss was measured 6\u2003wk after <jats:italic>P.\u00a0gingivalis<\/jats:italic> infection.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The presence of <jats:italic>S.\u00a0cristatus<\/jats:italic> CC5A, but not its <jats:italic>arcA<\/jats:italic> mutant, attenuated <jats:italic>P.\u00a0gingivalis<\/jats:italic> colonization in the murine oral cavity. In addition, <jats:italic>P.\u00a0gingivalis<\/jats:italic>\u2010induced alveolar bone loss was significantly lower in mice initially infected with <jats:italic>S.\u00a0cristatus<\/jats:italic> CC5A than in those infected with the <jats:italic>arcA<\/jats:italic> mutant.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> This study provides direct evidence that <jats:italic>S.\u00a0cristatus<\/jats:italic> ArcA has an inhibitory effect on <jats:italic>P.\u00a0gingivalis<\/jats:italic> colonization, which may in turn attenuate the pathogenicity of <jats:italic>P.\u00a0gingivalis.<\/jats:italic><\/jats:p>","DOI":"10.1111\/j.1600-0765.2012.01469.x","type":"journal-article","created":{"date-parts":[[2012,3,27]],"date-time":"2012-03-27T01:02:12Z","timestamp":1332810132000},"page":"578-583","source":"Crossref","is-referenced-by-count":40,"title":["<i>Streptococcus cristatus<\/i> ArcA interferes with <i>Porphyromonas gingivalis<\/i> pathogenicity in mice"],"prefix":"10.1111","volume":"47","author":[{"given":"H.","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u2010Y.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.511998"},{"key":"e_1_2_6_3_2","first-page":"16","article-title":"Periodontal inflammation: from gingivitis to systemic disease?","volume":"25","author":"Scannapieco FA","year":"2004","journal-title":"Compend Contin Educ Dent"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.grf.0000141894.85221.f7"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00107.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_6_7_2","first-page":"2713","article-title":"Detection of fimbriae and fimbrial antigens on the oral anaerobe Bacteroides gingivalis by negative staining and serological methods","volume":"134","author":"Suzuki Y","year":"1988","journal-title":"J Gen Microbiol"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1177\/08959374880020021601","article-title":"Fimbriae from the oral anaerobe Bacteroides gingivalis: a screening of clinical isolates from various places","volume":"2","author":"Suzuki Y","year":"1988","journal-title":"Adv Dent Res"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4028-4033.1999"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.7705\/biomedica.v29i2.31"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2003.00065.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760040601"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.17.5266-5274.1991"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.4.2359"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1993.tb00573.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1244-1263.1998"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00797-06"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.4.1052-1059.1994"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.4.2349"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.1.313-316.1997"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.24.7067-7069.2000"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00072-09"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/009050-0"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01627-06"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.11.3504-3508.1999"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400512"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2837-2848.2006"},{"key":"e_1_2_6_28_2","first-page":"369","article-title":"Microbiology of the early colonization of human enamel and root surfaces in vivo","volume":"95","author":"Nyvad B","year":"1987","journal-title":"Scand J Dent Res"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01631-09"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-6-1627"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00284-10"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.1.119"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345880670061501"},{"key":"e_1_2_6_34_2","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1128\/iai.62.5.2129-2131.1994","article-title":"Colonization of the murine oral cavity by Streptococcus gordonii","volume":"62","author":"Loach DM","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.2928-2934.2001"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2012.01469.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2012.01469.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T03:16:48Z","timestamp":1697253408000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2012.01469.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,26]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["10.1111\/j.1600-0765.2012.01469.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2012.01469.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,26]]}}}