{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:29:41Z","timestamp":1788992981628,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,28]],"date-time":"2012-03-28T00:00:00Z","timestamp":1332892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p><jats:italic>Galassi F, Kaman WE, Anssari Moin D, van der Horst J, Wismeijer D, Crielaard W, Laine ML, Veerman ECI, Bikker FJ, Loos BG. Comparing culture, real\u2010time PCR and fluorescence resonance energy transfer technology for detection of<\/jats:italic> Porphyromonas gingivalis <jats:italic>in patients with or without peri\u2010implant infections. J Periodont Res 2012; 47: 616\u2013625. \u00a9 2012 John Wiley &amp; Sons A\/S<\/jats:italic><\/jats:p><jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> The aim of the study was to compare the detection of <jats:italic>Porphyromonas gingivalis<\/jats:italic> using a fluorescence resonance energy transfer (FRET) technology with commonly used diagnostic methods in salivary and subgingival plaque samples from subjects with dental implants. <jats:italic>P.\u00a0gingivalis<\/jats:italic> was considered as a marker for a pathogenic microbiota.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Ninety\u2010seven adult subjects were recruited, including periodontally healthy controls with no dental implants, implant controls with no peri\u2010implant disease and patients with peri\u2010implant disease. Saliva and subgingival\/submucosal plaque samples were collected from all subjects and were analyzed using culture, real\u2010time PCR and FRET technology employing <jats:italic>P.\u00a0gingivalis<\/jats:italic>\u2010specific substrates.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> It was found that the <jats:italic>P.\u00a0gingivalis\u2010<\/jats:italic>specific substrates were highly suitable for detecting the presence of <jats:italic>P.\u00a0gingivalis<\/jats:italic> in saliva and in subgingival plaque samples, showing comparable specificity to culture and real\u2010time PCR.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> We applied the FRET technology to detect <jats:italic>P.\u00a0gingivalis<\/jats:italic> in implant patients with or without an implant condition and in controls without implants. The technique seems suitable for detection of <jats:italic>P.\u00a0gingivalis<\/jats:italic> in both plaque and saliva samples. However, with all three techniques, <jats:italic>P.\u00a0gingivalis<\/jats:italic> was not very specific for peri\u2010implantitis cases. Future work includes fine\u2010tuning the FRET technology and also includes the development of a chair\u2010side application.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2012.01474.x","type":"journal-article","created":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T07:25:29Z","timestamp":1333092329000},"page":"616-625","source":"Crossref","is-referenced-by-count":25,"title":["Comparing culture, real\u2010time PCR and fluorescence resonance energy transfer technology for detection of <i>Porphyromonas gingivalis<\/i> in patients with or without peri\u2010implant infections"],"prefix":"10.1111","volume":"47","author":[{"given":"F.","family":"Galassi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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