{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T10:40:26Z","timestamp":1771843226550,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,7,4]],"date-time":"2005-07-04T00:00:00Z","timestamp":1120435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p><jats:bold>Background\/Purpose: <\/jats:bold> It is known that solar ultraviolet (UV) irradiation exerts multiple effects on mammalian skin tissues, one of which is the induction of local and systemic immunosuppression as well as inflammation. Tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) and other cytokines are suggested to play a role in these responses. Quantitative real\u2010time polymerase chain reaction (TaqMan RTPCR) was used to elucidate the effect of UVA and UVB irradiation on the expression of genes coding for TNF\u2010\u03b1, IL\u20101\u03b2, IL\u201010, FasL, matrilysin, TACE and furin in HaCaT cells over a 48\u2003h period (IL\u20101\u03b2, interleukin\u20101\u03b2; FasL, Fas ligand).<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> Cultured HaCaT cells were either sham irradiated (control) or exposed to UVA (2000 and 8000\u2003J\/m<jats:sup>2<\/jats:sup>) or UVB (200 and 2000\u2003J\/m<jats:sup>2<\/jats:sup>) radiation. RNA was extracted from cells at 0, 4, 8, 12, 16, 24, 48\u2003h post\u2010irradiation and reverse transcribed to generate cDNA for subsequent real\u2010time PCR amplification.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> Significant increases in the mRNA levels for all genes tested were detected in both UVA\u2010 and UVB\u2010irradiated HaCaT cells compared with control (sham\u2010irradiated) cells. TNF\u2010\u03b1 mRNA levels were immediately up\u2010regulated (0\u2003h) after irradiation, with maximal induction at 8\u2003h post 2000\u2003J\/m<jats:sup>2<\/jats:sup> UVA and 200\u2003J\/m<jats:sup>2<\/jats:sup> UVB irradiation, at 4\u2003h post 8000\u2003J UVA irradiation and at 48\u2003h post 2000\u2003J\/m<jats:sup>2<\/jats:sup> UVB irradiation. No correlation was observed between TNF\u2010\u03b1, TACE and furin mRNA induction in the different irradiated cohorts.<\/jats:p><jats:p><jats:bold>Conclusion: <\/jats:bold> Results suggest that time\u2010distinct gene induction of TNF\u2010\u03b1, furin, IL\u20101\u03b2 and matrilysin may be involved in UV\u2010induced cellular responses, but not for TACE. In general, mRNA induction was dose dependent at some time points post\u2010irradiation, but not throughout the whole time course tested. Our results show that quantitative real\u2010time PCR is a useful tool in the analysis of quantitative changes of mRNA levels in cultured HaCaT cells after UV exposure.<\/jats:p>","DOI":"10.1111\/j.1600-0781.2005.00162.x","type":"journal-article","created":{"date-parts":[[2005,7,4]],"date-time":"2005-07-04T16:48:51Z","timestamp":1120495731000},"page":"173-182","source":"Crossref","is-referenced-by-count":42,"title":["Gene expression profiles of TNF\u2010\u03b1, TACE, furin, IL\u20101\u03b2 and matrilysin in UVA\u2010 and UVB\u2010irradiated HaCat cells"],"prefix":"10.1111","volume":"21","author":[{"given":"Beata","family":"Skiba","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bronwyn","family":"Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terrence 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