{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T05:40:57Z","timestamp":1697089257551},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,6,26]],"date-time":"2007-06-26T00:00:00Z","timestamp":1182816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p><jats:bold>Background: <\/jats:bold> Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti\u2010tumour influence by extracorporeal photopheresis has been documented for cutaneous T\u2010cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens.<\/jats:p><jats:p><jats:bold>Purpose: <\/jats:bold> To determine the potential of a simple clinical photopheresis protocol for large\u2010scale development of mature DC.<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> A standard monocyte\u2010enriched leukapheresis preparation of 10<jats:sup>9<\/jats:sup>\u201310<jats:sup>10<\/jats:sup> cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T\u2010cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth medium. Cell surface lymphocyte, monocyte and DC markers were determined using multi\u2010colour flow cytometry.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> We find signs of activation of the CD14+ monocytes, as well as the appearance of a minor population of mature DC negative for CD14 but with strong CD83 expression.<\/jats:p><jats:p><jats:bold>Conclusions: <\/jats:bold> With a procedure appropriate for routine clinical use, a total number of 10<jats:sup>6<\/jats:sup>\u201310<jats:sup>7<\/jats:sup> DC ready for patient reinfusion can be prepared within 24\u2003h. Our findings indicate the need to further explore the capacity of photopheresis to stimulate cancer patients' anti\u2010tumour defence reaction.<\/jats:p>","DOI":"10.1111\/j.1600-0781.2007.00285.x","type":"journal-article","created":{"date-parts":[[2007,6,26]],"date-time":"2007-06-26T13:19:39Z","timestamp":1182863979000},"page":"113-119","source":"Crossref","is-referenced-by-count":3,"title":["Clinical\u2010scale generation of strongly CD83\u2010expressing dendritic cells using extracorporeal photopheresis"],"prefix":"10.1111","volume":"23","author":[{"given":"Anders","family":"Bredberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svante","family":"Jonsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Lindblom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Bj\u00f6rk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0502261"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1592"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1845"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(200002)9999:9999<::AID-IJC1073>3.0.CO;2-R"},{"key":"e_1_2_6_6_2","first-page":"476","article-title":"Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant cell loading","volume":"6","author":"Salskov\u2010Iversen M","year":"2006","journal-title":"J Immune Based Ther Vacc"},{"key":"e_1_2_6_7_2","first-page":"85","article-title":"Review","volume":"43","author":"Bladon J","year":"2006","journal-title":"extracorporeal photopheresis: a focus on apoptosis and cytokines"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00670.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00174-2"},{"key":"e_1_2_6_10_2","first-page":"1495","article-title":"Transimmunization for cutaneous T cell lymphoma","volume":"47","author":"Girardi M","year":"2006","journal-title":"a phase I study"},{"key":"e_1_2_6_11_2","first-page":"301","article-title":"The Danger model","volume":"296","author":"Matzinger P.","year":"2002","journal-title":"a renewed sense of self"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002371-200307000-00010"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/14653240410004934"}],"container-title":["Photodermatology, Photoimmunology &amp; Photomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0781.2007.00285.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0781.2007.00285.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T15:26:56Z","timestamp":1697038016000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0781.2007.00285.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,26]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1111\/j.1600-0781.2007.00285.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0781.2007.00285.x","archive":["Portico"],"relation":{},"ISSN":["0905-4383","1600-0781"],"issn-type":[{"value":"0905-4383","type":"print"},{"value":"1600-0781","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,26]]}}}