{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:04:06Z","timestamp":1758819846159},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,1,4]],"date-time":"2011-01-04T00:00:00Z","timestamp":1294099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:p><jats:bold>Background: <\/jats:bold> Vitiligo is a frequently acquired, hereditary disease, characterized by achromic macules due to the absence of melanocytes. In contrast with earlier studies, in which the main pathogenic role was attributed to anti\u2010melanocyte antibodies, recent papers have emphasized a role for CD8<jats:sup>+<\/jats:sup> cytotoxic T lymphocytes in melanocyte destruction. Fifteen percent of peripheral T cell express cutaneous lymphocyte\u2010associated antigen (CLA), responsible for skin\u2010homing T cell. Phototherapy is used to treat patients with generalized vitiligo and it has been shown to interfere with CLA<jats:sup>+<\/jats:sup> T cells in other skin diseases.<\/jats:p><jats:p><jats:bold>Objective: <\/jats:bold> To describe peripheral blood T cell subpopulations' frequency and ability to express the skin\u2010homing molecule (CLA) in patients with non\u2010segmental vitiligo, before and after photochemotherapy (PUVA).<\/jats:p><jats:p><jats:bold>Patients and Methods: <\/jats:bold> Twenty\u2010two patients with generalized and active spreading vitiligo were submitted to 30 PUVA\u20108MOP sessions. Lymphocyte immunophenotyping was performed by flow cytometry using anti\u2010CD3, anti\u2010CD8 and anti\u2010CLA monoclonal antibodies. Fifteen healthy volunteers, sex\u2010 and age\u2010matched, were included as a control group.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> CD8<jats:sup>+<\/jats:sup>\u2013CLA<jats:sup>+<\/jats:sup> T cells were significantly reduced in number in untreated vitiligo patients (<jats:italic>P<\/jats:italic>=0.008) when compared with control individuals, albeit with a more intense CLA expression (<jats:italic>P<\/jats:italic>=0.028). These findings were not altered after PUVA. No significant difference was noticed in CD4\/CD8 ratios nor in CD4\u2013CLA<jats:sup>+<\/jats:sup> T cell numbers between vitiligo patients and controls, both before and after PUVA.<\/jats:p><jats:p><jats:bold>Conclusions: <\/jats:bold> CD8\u2013CLA<jats:sup>+<\/jats:sup> T cells are reduced in peripheral blood of patients with non\u2010segmental vitiligo. This finding may be related to the previously reported increase of CD8<jats:sup>+<\/jats:sup> cells in both lesions and perilesional skin of these patients.<\/jats:p>","DOI":"10.1111\/j.1600-0781.2010.00563.x","type":"journal-article","created":{"date-parts":[[2011,1,4]],"date-time":"2011-01-04T09:57:46Z","timestamp":1294135066000},"page":"40-44","source":"Crossref","is-referenced-by-count":5,"title":["Reduction of skin\u2010homing cytotoxic T cells (CD8<sup>+<\/sup>\u2013CLA<sup>+<\/sup>) in patients with vitiligo"],"prefix":"10.1111","volume":"27","author":[{"given":"Daniela Pereira","family":"Antelo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Absalom Lima","family":"Filgueira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Marcos 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