{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T03:10:59Z","timestamp":1761621059004},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,3,10]],"date-time":"2011-03-10T00:00:00Z","timestamp":1299715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:p><jats:bold>Background: <\/jats:bold> Clinical studies have demonstrated that photodynamic therapy (PDT) for hyperplastic dermatosis results in a beneficial outcome. Hypertrophic scar (HS) is a pathological process characterized by fibroblastic hyperproliferation. However, it is unclear whether photochemical interactions between PDT and fibroblasts contribute to a beneficial outcome. To investigate the primary photochemical effects of PDT, we studied the efficacy of 630\u2003nm PDT on human fibroblasts from HS using hematoporphyrin monomethyl ether (HMME) as a photosensitizer.<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> Fibroblasts were cultured from nontreated HSs, and cells at passage 4\u20136 were used for the experiments. Morphological and biochemical changes in fibroblasts were assessed by Hoechst 33258 staining and annexin V\u2010FITC\/PI flow cytometry (FCM). Caspase\u20103 activity assay and immunofluorescence staining were performed to investigate caspase\u20103 expression in fibroblasts.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> The morphological features of cell apoptosis were viewed under a fluorescent microscope by Hoechst 33258 staining. FCM indicated that the apoptotic rate was significantly increased after HMME\u2013PDT, and caspase\u20103 activity was observed.<\/jats:p><jats:p><jats:bold>Conclusions: <\/jats:bold> Low\u2010level exposure to 630\u2003nm PDT mediated by HMME appears to induce fibroblast apoptosis and stimulate caspase\u20103 activation. However, the effect of HMME\u2013PDT on fibroblasts needs further investigation to determine its therapeutic potential for HS.<\/jats:p>","DOI":"10.1111\/j.1600-0781.2011.00577.x","type":"journal-article","created":{"date-parts":[[2011,3,11]],"date-time":"2011-03-11T01:07:27Z","timestamp":1299805647000},"page":"90-96","source":"Crossref","is-referenced-by-count":18,"title":["Effect of hematoporphyrin monomethyl ether\u2010mediated photodynamic therapy on hypertrophic scar 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