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In higher plants and other photoautotrophic organisms, contradictory results for melatonin content for samples from the same species show that further improvement of methods for reliable quantification is required. In the present study, melatonin was quantified in tomatoes, ginger and the marine green macroalga, <jats:italic>Ulva lactuca<\/jats:italic>, after extraction with three different extraction methods based on ether, acetone or perchloric acid. Melatonin was determined by enzyme\u2010linked immunosorbent assay (ELISA) in high\u2010performance liquid chromatography (HPLC)\u2010purified extracts. The same HPLC system used for purification of extracts was used for parallel quantifications after derivatization of melatonin under alkaline conditions in the presence of hydrogen peroxide (HPLC\u2010PD). Both quantification methods gave similar results with a high correlation [<jats:italic>f<\/jats:italic>(<jats:italic>x<\/jats:italic>)\u2003=\u20030.99<jats:italic>x<\/jats:italic>\u2003+\u20033.01; <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2003=\u20030.99]. In ginger, the melatonin concentration was below 5\u2003pg\/g (fresh weight, f.w.), whereas in tomatoes about 1200\u2003pg\/g (f.w.) were found, and in the green alga, <jats:italic>U. lactuca<\/jats:italic>, approximately 12\u2003pg\/g (f.w.). Taking into account the recovery rates for synthetic melatonin added prior to extraction, no substantial differences were observed in melatonin quantification between different extraction methods. The demonstrated methods based on HPLC purification and subsequent quantification by ELISA and HPLC\u2010PD allow highly sensitive melatonin determinations in diverse photoautotrophic organisms with a low risk of overestimations by false\u2010positive results.<\/jats:p>","DOI":"10.1111\/j.1600-079x.2006.00348.x","type":"journal-article","created":{"date-parts":[[2006,6,9]],"date-time":"2006-06-09T10:26:51Z","timestamp":1149848811000},"page":"157-165","source":"Crossref","is-referenced-by-count":123,"title":["Quantification of melatonin in phototrophic organisms"],"prefix":"10.1111","volume":"41","author":[{"given":"Carsten","family":"Pape","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"L\u00fcning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,6,22]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01543a060"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01531a060"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(91)90087-9"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/1043-2760(91)90055-R"},{"key":"e_1_2_5_6_2","first-page":"147","article-title":"Melatonin involvement in oxidative stress","volume":"29","author":"Ianas O","year":"1991","journal-title":"Rom J Endocrinol"},{"key":"e_1_2_5_7_2","first-page":"57","article-title":"Melatonin: a potent, endogenous hydroxyl radical scavenger","volume":"1","author":"Tan DX","year":"1993","journal-title":"Endocr J"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:27:2:119"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(94)00532-X"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000014635"},{"key":"e_1_2_5_11_2","first-page":"171","article-title":"Melatonin: mechanisms and actions as an antioxidant","volume":"24","author":"Reiter RJ","year":"2000","journal-title":"Curr Top Biophys"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-079X.2003.00092.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01134354"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01923945"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1997.tb00310.x"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1508\/cytologia.64.209"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1995.tb00147.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/07420529209064535"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1997.tb00353.x"},{"key":"e_1_2_5_20_2","first-page":"86","volume-title":"Biological Rhythms and Antioxidative Protection","author":"Antol\u00edn I","year":"1997"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-46814-X_45"},{"key":"e_1_2_5_22_2","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1242\/jcs.5.3.745","article-title":"Regulation of mitosis. 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