{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:00:55Z","timestamp":1790917255296,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,6,22]],"date-time":"2006-06-22T00:00:00Z","timestamp":1150934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> The rod outer segment (ROSg) membranes are essentially lipoprotein complexes. Rhodopsin, the major integral protein of ROSg, is surrounded by phospholipids highly enriched in docosahexaenoic acid (22:6 n3). This fluid environment plays an important role for conformational changes after photo\u2010activation. Thus, ROSg membranes are highly susceptible to oxidative damage. Melatonin synthesized in the pineal gland, retina and other tissues is a free radical scavenger. The principal aim of this work was to study the changes in the ROSg membranes isolated from bovine retina submitted to nonenzymatic lipid peroxidation (ascorbate\u2010Fe<jats:sup>2+<\/jats:sup> induced), during different time intervals (0\u2013180\u2003min). Oxidative stress was monitored by increase in the chemiluminescence and fatty acid alterations. In addition we studied the in vitro protective effect of 5\u2003m<jats:sc>m<\/jats:sc> melatonin. The total cpm originated from light emission (chemiluminescence) was found to be lower in those membranes incubated in the presence of melatonin. The docosahexaenoic acid content decreased considerably when the membranes were exposed to oxidative damage. This reduction was from 35.5\u2003\u00b1\u20032.9% in the native membranes to 12.65\u2003\u00b1\u20031.86% in those peroxidized during 180\u2003min. In the presence of 5\u2003m<jats:sc>m<\/jats:sc> melatonin we observed a content preservation of 22:6 n3 (23.85\u2003\u00b1\u20032.77%) at the same time of peroxidation. Simultaneously the alterations of membrane proteins under oxidative stress were studied using sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE). Loss of protein sulfhydryl groups and increased incorporation of carbonyl groups were utilized as biomarkers of protein oxidation. In membranes exposed to Fe<jats:sup>2+<\/jats:sup>\u2010ascorbate, we observed a decrease of protein thiols from 50.9\u2003\u00b1\u20033.38 in native membranes to 1.72\u2003\u00b1\u20032.81\u2003nmol\/mg of protein after 180\u2003min of lipid peroxidation associated with increased incorporation of carbonyl groups into proteins from 7.20\u2003\u00b1\u20032.50 to 12.50\u2003\u00b1\u20031.12\u2003nmol\/mg of protein. In the SDS\u2010PAGE we observed a decrease in the content of all the proteins, mainly rhodopsin, as a consequence of peroxidation. Melatonin, prevent both lipid peroxidation and protein oxidation.<\/jats:p>","DOI":"10.1111\/j.1600-079x.2006.00352.x","type":"journal-article","created":{"date-parts":[[2006,6,22]],"date-time":"2006-06-22T09:55:13Z","timestamp":1150970113000},"page":"201-210","source":"Crossref","is-referenced-by-count":23,"title":["Lipid\u2013protein modifications during ascorbate\u2010Fe<sup>2+<\/sup> peroxidation of photoreceptor membranes: protective effect of melatonin"],"prefix":"10.1111","volume":"41","author":[{"given":"Margarita H.","family":"Guajardo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Terrasa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Catal\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,6,22]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2004.05.012"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7827(83)90004-8"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(02)00121-X"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.22.3.229.5511"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2005.00304.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02536182"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-9877(02)00333-X"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(00)00117-0"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx00031a001"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(03)00185-7"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079X.2003.02112.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2003_3637"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb09920.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(80)67038-4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(79)90102-4"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(72)25041-8"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(94)33040-9"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/01926230290166724"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005425213253"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2006.02.010"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1159\/000267901"},{"key":"e_1_2_6_26_2","first-page":"2091","article-title":"Lipid peroxidation in rod outer segments. 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