{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:47:46Z","timestamp":1761630466879},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,10,23]],"date-time":"2007-10-23T00:00:00Z","timestamp":1193097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Impaired Ca<jats:sup>2+<\/jats:sup> homeostasis and smooth muscle contractility co\u2010exist in acute cholecystitis (AC) leading to gallbladder dysfunction. There is no pharmacological treatment for this pathological condition. Our aim was to evaluate the effects of melatonin treatment on Ca<jats:sup>2+<\/jats:sup> signaling pathways and contractility altered by cholecystitis. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> was determined by epifluorescence microscopy in fura\u20102 loaded isolated gallbladder smooth muscle cells, and isometric tension was recorded from gallbladder muscle strips. Malondialdehyde (MDA) and reduced glutathione (GSH) contents were determined by spectrophotometry and cycloxygenase\u20102 (COX\u20102) expression was quantified by western blot. Melatonin was tested in two experimental groups, one of which underwent common bile duct ligation for 2\u2003days and another that was later de\u2010ligated for 2\u2003days. Inflammation\u2010induced impairment of Ca<jats:sup>2+<\/jats:sup> responses to cholecystokinin and caffeine were recovered by melatonin treatment (30\u2003mg\/kg). This treatment also ameliorated the detrimental effects of AC on Ca<jats:sup>2+<\/jats:sup> influx through both L\u2010type and capacitative Ca<jats:sup>2+<\/jats:sup> channels, and it was effective in preserving the pharmacological phenotype of these channels. Despite its effects on Ca<jats:sup>2+<\/jats:sup> homeostasis, melatonin did not improve contractility. After de\u2010ligation, Ca<jats:sup>2+<\/jats:sup> influx and contractility were still impaired, but both were recovered by melatonin. These effects of melatonin were associated to a reduction of MDA levels, an increase in GSH content and a decrease in COX\u20102 expression. These findings indicate that melatonin restores Ca<jats:sup>2+<\/jats:sup> homeostasis during AC and resolves inflammation. In addition, this indoleamine helps in the subsequent recovery of functionality.<\/jats:p>","DOI":"10.1111\/j.1600-079x.2007.00520.x","type":"journal-article","created":{"date-parts":[[2007,10,26]],"date-time":"2007-10-26T23:25:14Z","timestamp":1193441114000},"page":"250-260","source":"Crossref","is-referenced-by-count":12,"title":["Protective effect of melatonin on Ca<sup>2+<\/sup> homeostasis and contractility in acute cholecystitis"],"prefix":"10.1111","volume":"44","author":[{"given":"Pedro J.","family":"Gomez\u2010Pinilla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro J.","family":"Camello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda 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