{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:13:18Z","timestamp":1773285198067,"version":"3.50.1"},"reference-count":9,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,3,31]],"date-time":"2008-03-31T00:00:00Z","timestamp":1206921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:p><jats:bold>Background\/purpose: <\/jats:bold> The wound healing process involves unexplained mechanisms. An aberration in this process is known to cause dermal disorders such as keloid or hypertrophic scars, but the mechanism by which these scars are formed remains to be elucidated. Here we examined the usefulness of a non\u2010invasive optical imaging device to clarify mechanisms of wound healing and of scar formation.<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> An 8\u2003mm experimental wound was made in the forearms of six subjects by a suction blister method. To observe chronological changes associated with wound healing, horizontal cross\u2010sectional images were non\u2010invasively obtained of the wounded area from the skin surface down to 129\u2003\u03bcm below at 21.5\u2003\u03bcm intervals using <jats:italic>in vivo<\/jats:italic> laser confocal scanning microscopy (LCSM).<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> The wounds were covered with a new epidermis by week 2, at which time the dermal papilla count decreased while the thickness from the skin surface to the apex of the dermal papilla increased. The count and the thickness returned to the initial levels when the wound was healed. In two out of six subjects, fibrous tissues were observed in the upper dermis, whereas in one other subject, melanocyte\u2010like dendritic cells were observed in the epidermis\u2013dermis border in later phases of wound healing.<\/jats:p><jats:p><jats:bold>Conclusion: <\/jats:bold> This non\u2010invasive method using <jats:italic>in vivo<\/jats:italic> LCSM revealed chronological changes in the dermis and epidermis during wound healing. In addition, although a scar was not formed in any of study subjects, this microscopy revealed aspects similar to the fibrous tissue overgrowth or to melanocyte migration, both of which may relate to wound healing. These results indicate the usefulness of this non\u2010invasive method in studies of wound healing and of scar formation.<\/jats:p>","DOI":"10.1111\/j.1600-0846.2008.00308.x","type":"journal-article","created":{"date-parts":[[2008,3,31]],"date-time":"2008-03-31T09:30:06Z","timestamp":1206955806000},"page":"436-439","source":"Crossref","is-referenced-by-count":11,"title":["Changes of human skin in subepidermal wound healing process"],"prefix":"10.1111","volume":"14","author":[{"given":"Keiichi","family":"Sugata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Kitahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Takema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,10,10]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"946","article-title":"In vivo confocal scanning laser microscopy of human skin","volume":"104","author":"Rajadhyaksha M","year":"1995","journal-title":"melanin provides strong contrast"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-202x.2001.01337.x"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(64)92011-2"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.1968.15"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700786"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1996.03890360039008"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23562.x"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.1577349"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.1648646"}],"container-title":["Skin Research and Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0846.2008.00308.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0846.2008.00308.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T19:13:29Z","timestamp":1696965209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0846.2008.00308.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,10]]},"references-count":9,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["10.1111\/j.1600-0846.2008.00308.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0846.2008.00308.x","archive":["Portico"],"relation":{},"ISSN":["0909-752X","1600-0846"],"issn-type":[{"value":"0909-752X","type":"print"},{"value":"1600-0846","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,10]]}}}