{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T10:54:47Z","timestamp":1777114487938,"version":"3.51.4"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,6,22]],"date-time":"2012-06-22T00:00:00Z","timestamp":1340323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Research and Technology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"Background\/purposes<\/jats:title>Fluorescence emission from in vivo<\/jats:italic> cutaneous melanin was recently detected under near\u2010infrared (NIR<\/jats:styled-content>) excitation by our group. We then built a prototype NIR<\/jats:styled-content> autofluorescence imaging system to observe and characterize the melanin distribution in human skin. In this article, we reported a new setup of NIR<\/jats:styled-content> fluorescence imaging system and calibration methods to optimize the system for better clinical feasibility and clearer image.<\/jats:p><\/jats:sec>Methods<\/jats:title>The imaging system was designed to perform both fluorescence and reflectance imaging with a 785\u2010nm fiber\u2010coupled laser source. The illumination light was purified by a 785\u2010nm bandpass filter for fluorescence excitation; while the spontaneous components were selected by a longpass filter for NIR<\/jats:styled-content> reflectance imaging. A hand\u2010controlled filter wheel was used to switch these two filters for different imaging modes. A dichroic filter was used to guide the illuminating light onto the skin surface for excitation. Reflectance and fluorescence signals were collected sequentially by a NIR<\/jats:styled-content> optimized CCD<\/jats:styled-content> camera. The captured images were calibrated by the reflectance images of a standard reflectance disk for non\u2010uniform illuminations and light collection efficiencies.<\/jats:p><\/jats:sec>Results<\/jats:title>The clinical results demonstrated that NIR<\/jats:styled-content> fluorescence intensities and distribution patterns vary among lesion types. It was also confirmed that pigmented skin lesions emitted higher NIR<\/jats:styled-content> fluorescence than the surrounding normal skin due to the presentation of higher concentrations of cutaneous melanin within the lesions.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>NIR<\/jats:styled-content> autofluorescence imaging system could be utilized as a powerful tool for visualizing melanin distribution in pigmented skin lesions and as a potential method for aiding melanoma detection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0846.2012.00632.x","type":"journal-article","created":{"date-parts":[[2012,6,22]],"date-time":"2012-06-22T18:31:19Z","timestamp":1340389879000},"page":"20-26","source":"Crossref","is-referenced-by-count":40,"title":["In vivo<\/i> near\u2010infrared autofluorescence imaging of pigmented skin lesions: methods, technical improvements and preliminary clinical results"],"prefix":"10.1111","volume":"19","author":[{"given":"Shuang","family":"Wang","sequence":"first","affiliation":[{"name":"Imaging Unit \u2013 Integrative Oncology Department British Columbia Cancer Agency Research Centre Vancouver BC Canada"},{"name":"Laboratory for Advanced Medical Photonics Photomedicine Institute Department of Dermatology and Skin Science University of British Columbia and Vancouver Coastal Health Research Institute Vancouver BC Canada"},{"name":"Institue of Photonics and Photon\u2010Technology and Department of Physics Northwest University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Imaging Unit \u2013 Integrative Oncology Department British Columbia Cancer Agency Research Centre Vancouver BC Canada"},{"name":"Laboratory for Advanced Medical Photonics Photomedicine Institute Department of Dermatology and Skin Science University of British Columbia and Vancouver Coastal Health Research Institute Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harvey","family":"Lui","sequence":"additional","affiliation":[{"name":"Imaging Unit \u2013 Integrative Oncology Department British Columbia Cancer Agency Research Centre Vancouver BC Canada"},{"name":"Laboratory for Advanced Medical Photonics Photomedicine Institute Department of Dermatology and Skin Science University of British Columbia and Vancouver Coastal Health Research Institute Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingli","family":"He","sequence":"additional","affiliation":[{"name":"Institue of Photonics and Photon\u2010Technology and Department of Physics Northwest University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haishan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Imaging Unit \u2013 Integrative Oncology Department British 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