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To investigate the role of lipid rafts in secretory granule exocytosis, we examined the raft association of SNARE proteins and SNARE complexes in rat basophilic leukemia (RBL) mast cells. The SNARE protein SNAP\u201023 co\u2010localized with a lipid raft marker and was present in detergent\u2010insoluble lipid raft microdomains in RBL cells. By contrast, only small amounts (&lt;20%) of the plasma membrane SNARE syntaxin 4 or the granule\u2010associated SNARE vesicle\u2010associated membrane protein (VAMP)\u20102 were present in these microdomains. Despite this, essentially all syntaxin 4 and most of VAMP\u20102 in these rafts were present in SNARE complexes containing SNAP\u201023, while essentially none of these complexes were present in nonraft membranes. Whereas SNAP\u201023 is membrane anchored by palmitoylation, the association of the transmembrane protein syntaxin 4 with lipid rafts was because of its binding to SNAP\u201023. After stimulating mast cells exocytosis, the amount of syntaxin 4 and VAMP\u20102 present in rafts increased twofold, and these proteins were now present in raft\u2010associated phospho\u2010SNAP\u201023\/syntaxin 4\/VAMP\u20102 complexes, revealing differential association of SNARE fusion complexes during the process of regulated exocytosis.<\/jats:bold><\/jats:p>","DOI":"10.1111\/j.1600-0854.2006.00490.x","type":"journal-article","created":{"date-parts":[[2006,9,19]],"date-time":"2006-09-19T11:01:17Z","timestamp":1158663677000},"page":"1482-1494","source":"Crossref","is-referenced-by-count":89,"title":["Ternary SNARE Complexes Are Enriched in Lipid Rafts during Mast Cell Exocytosis"],"prefix":"10.1111","volume":"7","author":[{"given":"Niti","family":"Puri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul 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