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Membrane\u2010associated clathrin\u2010coated structures (CCS) constitutively appeared at the peripheral filopodial membrane, moved centripetally while growing in intensity, before being eventually endocytosed within a few tens of seconds. This directed CCS traffic was independent of microtubules but could be blocked by latrunculin A. Taking advantage of available mutants of<jats:italic> Drosophila<\/jats:italic>, we expressed clathrin\u2013EGFP in<jats:italic> wasp<\/jats:italic> and<jats:italic> shibire<\/jats:italic> mutant backgrounds to study the role of actin and dynamin in CCS dynamics and CME in hemocytes. We show that actin plays an essential role in CME in these cells, and that actin and dynamin act at the same stage, but independent of each other.<jats:italic> Drosophila melanogaster<\/jats:italic> hemocytes proved to be a promising model system to uncover the molecular events during CME in combining live\u2010cell imaging and genetic analysis.<\/jats:bold><\/jats:p>","DOI":"10.1111\/j.1600-0854.2006.00492.x","type":"journal-article","created":{"date-parts":[[2006,10,2]],"date-time":"2006-10-02T13:27:28Z","timestamp":1159795648000},"page":"1614-1627","source":"Crossref","is-referenced-by-count":25,"title":["Imaging Clathrin Dynamics in<i> Drosophila melanogaster<\/i> Hemocytes Reveals a Role for Actin in Vesicle Fission"],"prefix":"10.1111","volume":"7","author":[{"given":"Olexiy","family":"Kochubey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amitabha","family":"Majumdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurgen","family":"Klingauf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(01)02082-7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90087-C"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00097-0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.111401.093127"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1313"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35044540"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2005.00280.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb837"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-09-0774"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02444"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00356"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00637"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200205086"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.1.1"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.97.2.499"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.4.915"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C300390200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2005.00329.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400130107"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.1.135"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.08.017"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.03.015"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2003.00100.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402871200"},{"key":"e_1_2_6_26_2","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1242\/jcs.110.19.2441","article-title":"Microtubule depolymerization inhibits clathrin coated\u2010pit internalization in non\u2010adherent cell lines while interleukin 2 endocytosis is not affected","volume":"110","author":"Subtil A","year":"1997","journal-title":"J Cell Sci"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.06.016"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-004-0403-6"},{"key":"e_1_2_6_29_2","volume":"67","author":"Agero U","year":"2003","journal-title":"Cell surface fluctuations studied with defocusing microscopy. 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