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Most sterol accumulation studies in NPC cells have been carried out using low\u2010density lipoprotein (LDL) as the sterol source, and it has been shown that sterol efflux from late endosomes is impaired in NPC cells. In this study, we used a fluorescent sterol analog, dehydroergosterol, which can be quickly and efficiently delivered to the plasma membrane. Thus, we were able to study the trafficking kinetics of the non\u2010LDL\u2010derived sterol pool, and we found that dehydroergosterol accumulates in the storage organelles over the course of several hours in NPC cells. We also found that dialkylindocarbocyanine lipid\u2010mimetic analogs that recycle efficiently from early endosomes in wild\u2010type cells are targeted to late endosomal organelles in NPC cells, and transferrin receptors recycle slowly and inefficiently in NPC cells. These data are consistent with multiple trafficking defects in both early and late endosomes in NPC cells.<\/jats:bold><\/jats:p>","DOI":"10.1111\/j.1600-0854.2006.00513.x","type":"journal-article","created":{"date-parts":[[2006,12,7]],"date-time":"2006-12-07T11:46:15Z","timestamp":1165491975000},"page":"130-141","source":"Crossref","is-referenced-by-count":55,"title":["Sterol, Protein and Lipid Trafficking in Chinese Hamster Ovary Cells with Niemann\u2010Pick Type C1 Defect"],"prefix":"10.1111","volume":"8","author":[{"given":"Nina H.","family":"Pipalia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushmita","family":"Mukherjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick 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