{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T07:05:21Z","timestamp":1793171121020,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,11,6]],"date-time":"2007-11-06T00:00:00Z","timestamp":1194307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>\n                    <jats:bold>Sorting nexin 9 (SNX9) functions at the interface between membrane remodeling and the actin cytoskeleton. In particular, SNX9 links membrane binding to potentiation of N\u2010WASP and dynamin GTPase activities. SNX9 is one of a growing number of proteins that contain two lipid\u2010binding domains, a phox homology (PX) and a Bin1\/Amphiphysin\/RVS167 (BAR) domain, and localizes to diverse membranes that are enriched in different phosphoinositides. Here, we investigate the mechanism by which SNX9 functions at these varied membrane environments. We show that SNX9 has low\u2010lipid\u2010binding affinity and harnesses a broad range of phosphoinositides to synergistically enhance both dynamin and N\u2010WASP activities. We introduced point mutations in either the PX domain, BAR domain or both that are predicted to disrupt their functions and examined their respective roles in lipid\u2010binding, and dynamin and N\u2010WASP activation. We show that the broad lipid specificity of SNX9 is not because of independent and additive contributions by individual domains. Rather, the two domains appear to function in concert to confer lipid\u2010binding and SNX9\u2019s membrane active properties. We also demonstrate that the two domains are differentially required for full SNX9 activity in N\u2010WASP and dynamin regulation, and for localization of SNX9 to clathrin\u2010coated pits and dorsal ruffles. In total, our results suggest that SNX9 can integrate signals from varied lipids through two domains to direct membrane remodeling events at multiple cellular locations.<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1111\/j.1600-0854.2007.00675.x","type":"journal-article","created":{"date-parts":[[2007,11,15]],"date-time":"2007-11-15T20:21:39Z","timestamp":1195158099000},"page":"133-146","source":"Crossref","is-referenced-by-count":95,"title":["SNX9 Activities are Regulated by Multiple Phosphoinositides Through both PX and BAR Domains"],"prefix":"10.1111","volume":"9","author":[{"given":"Defne","family":"Yarar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark C.","family":"Surka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilyn C.","family":"Leonard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra L.","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,11,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092586"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2006.04.011"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610543104"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407430200"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-11-1016"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.04.014"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700283200"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05185"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307334200"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.08.004"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"2898","DOI":"10.1038\/sj.emboj.7601174","article-title":"Mechanism of endophilin N\u2010BAR domain\u2010mediated membrane curvature","volume":"21","author":"Gallop JL","year":"2006","journal-title":"Embo J"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.42.27725"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)29107-1"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/374190a0"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.11.054"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.09.077"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107080200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/88591"},{"key":"e_1_2_6_20_2","unstructured":"KaanH SondermannH.Structure of BAR and PX Domains of Sorting Nexin 9 Reveals Cooperativity in Membrane Binding;2007.http:\/\/ecommonslibrarycornelledu\/handle\/1813\/7893(Cornell University)."},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601771"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)04043-7"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601491"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)29106-X"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407007200"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-2720.2004.01408.x"},{"key":"e_1_2_6_27_2","article-title":"The PX\u2010BAR membrane\u2010remodeling unit of sorting nexin 9","author":"Pylypenko Olena","journal-title":"EMBO 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