{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:24:31Z","timestamp":1791149071887,"version":"4.1.0"},"reference-count":71,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,2,8]],"date-time":"2011-02-08T00:00:00Z","timestamp":1297123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      Phagosome maturation is defined as the process by which phagosomes fuse sequentially with endosomes and lysosomes to acquire an acidic pH and hydrolases that degrade ingested particles. While the essential role of actin cytoskeleton remodeling during particle internalization is well established, its role during the later stages of phagosome maturation remains largely unknown. We have previously shown that purified mature phagosomes assemble F\u2010actin at their membrane, and that the ezrin\u2010radixin\u2010moesin (ERM) proteins ezrin and moesin participate in this process. Moreover, we provided evidence that actin assembly on purified phagosomes stimulates their fusion with late endocytic compartments\n                      <jats:italic>in vitro<\/jats:italic>\n                      . In this study, we further investigated the role of ezrin in phagosome maturation. We engineered a structurally open form of ezrin and demonstrated that ezrin binds directly to the actin assembly promoting factor N\u2010WASP (Neural Wiskott\u2010Aldrich Syndrome Protein) by its FERM domain. Using a cell\u2010free system, we found that ezrin stimulates F\u2010actin assembly on purified phagosomes by recruiting the N\u2010WASP\u2013Arp2\/3 machinery. Accordingly, we showed that the down\u2010regulation of ezrin activity in macrophages by a dominant\u2010negative approach caused reduced F\u2010actin accumulation on maturing phagosomes. Furthermore, using fluorescence and electron microscopy, we found that ezrin is required for the efficient fusion between phagosomes and lysosomes. Live\u2010cell imaging analysis supported the notion that ezrin is necessary for the fusogenic process itself, promoting the transfer of the lysosome content into the phagosomal lumen.\n                    <\/jats:bold>\n                  <\/jats:p>","DOI":"10.1111\/j.1600-0854.2011.01158.x","type":"journal-article","created":{"date-parts":[[2011,1,6]],"date-time":"2011-01-06T18:15:02Z","timestamp":1294337702000},"page":"421-437","source":"Crossref","is-referenced-by-count":74,"title":["Ezrin Promotes Actin Assembly at the Phagosome Membrane and Regulates Phago\u2010Lysosomal Fusion"],"prefix":"10.1111","volume":"12","author":[{"given":"Sabrina","family":"Marion","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eik","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Holzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Le 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