{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:52:22Z","timestamp":1792230742099,"version":"4.4.0"},"reference-count":63,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,7,29]],"date-time":"2012-07-29T00:00:00Z","timestamp":1343520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p><jats:bold>Human catalase forms a 240\u2010kDa tetrameric complex and degrades <jats:styled-content style=\"fixed-case\">H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub><\/jats:styled-content> in peroxisomes. Human catalase is targeted to peroxisomes by the interaction of its peroxisomal targeting signal type 1 (<jats:styled-content style=\"fixed-case\">PTS1<\/jats:styled-content>)\u2010like <jats:styled-content style=\"fixed-case\">KANL<\/jats:styled-content> sequence with the cytosolic <jats:styled-content style=\"fixed-case\">PTS1<\/jats:styled-content> receptor Pex5p. We show herein that human catalase tetramers are formed in the cytoplasm and that the expression of a <jats:styled-content style=\"fixed-case\">PTS<\/jats:styled-content> signal on each of the four subunits is not necessary for peroxisomal transport. We previously demonstrated that a Pex5p mutant defective in binding to Pex13p, designated Pex5p(Mut234), imports typical <jats:styled-content style=\"fixed-case\">PTS1<\/jats:styled-content>\u2010type proteins but not catalase. This impaired catalase import is not rescued by replacing its C\u2010terminal <jats:styled-content style=\"fixed-case\">KANL<\/jats:styled-content> sequence with a typical <jats:styled-content style=\"fixed-case\">PTS1<\/jats:styled-content> sequence, <jats:styled-content style=\"fixed-case\">SKL<\/jats:styled-content>, indicating that the failure of catalase import in Mut234\u2010expressing cells is not due to its weak <jats:styled-content style=\"fixed-case\">PTS1<\/jats:styled-content>. In contrast, several enzymatically inactive and monomeric mutants of catalase are efficiently imported in Mut234\u2010expressing cells. Moreover, trimeric chloramphenicol acetyltransferase (<jats:styled-content style=\"fixed-case\">CAT<\/jats:styled-content>) harboring <jats:styled-content style=\"fixed-case\">SKL<\/jats:styled-content> is not imported in Pex5p(Mut234)\u2010expressing cells, but <jats:styled-content style=\"fixed-case\">CAT\u2010SKL<\/jats:styled-content> trimers are transported to peroxisomes in the wild\u2010type cells. These findings suggest that the Pex5p\u2013Pex13p interaction likely plays a pivotal role in the peroxisomal import of folded and oligomeric proteins.<\/jats:bold><\/jats:p>","DOI":"10.1111\/j.1600-0854.2012.01391.x","type":"journal-article","created":{"date-parts":[[2012,7,2]],"date-time":"2012-07-02T18:15:07Z","timestamp":1341252907000},"page":"1364-1377","source":"Crossref","is-referenced-by-count":36,"title":["Pex5p Imports Folded Tetrameric Catalase by Interaction with Pex13p"],"prefix":"10.1111","volume":"13","author":[{"given":"Hidenori","family":"Otera","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Sciences, Graduate School of Systems Life Sciences Kyushu University  Fukuoka 812\u20108581 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukio","family":"Fujiki","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, Graduate School of Systems Life Sciences Kyushu University  Fukuoka 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