{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:06:49Z","timestamp":1758924409445},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,9,6]],"date-time":"2011-09-06T00:00:00Z","timestamp":1315267200000},"content-version":"vor","delay-in-days":5118,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>PROBLEM: Allopregnancy induces specific transient tolerance to paternal grafts, and we know that a low molecular weight material (\u201cfiltrate\u201d) present in a human placental supernatant can do so <jats:italic>in vitro<\/jats:italic> (specific unresponsiveness) as well as <jats:italic>in vivo<\/jats:italic>, such as when preventing grafl\u2010versus\u2010host reaction (GVH) produced by A cells injected into irradiated A \u00d7 B F1s recipients. We also know by studies carried out using specific anti\u2010V\u03b2\u2010specific stimulation as well as secondary and primary mixed lymphocyte reaction in major histocompatibility complex (MHC) only incompatible combinations that the material acts by inducing T cell anergy rather than clonal deletion. We explored the mechanism of such an anergy, which we knew was not dependent on calcium fluxes, cyclic adenosine monophosphate (cAMP) levels, or PkC by studies of protein phosphorylation. Having observed in previous studies that expression of T cell reactivity (TcR) in anergic cells was enhanced, but that the numbers of cells expressing a given V\u03b2 after specific stimulation in the presence of a filtrate was much higher than it should be, we monitored the receptor expression by fluorescence\u2010activated cell sorter (FACS).<\/jats:p><jats:p>METHOD OF STUDY: We used short\u2010term stimulation of the T\u2010cell\u2010derived Jurkat E6\u20101 cells by anti\u2010CD3 monoclonal antibody (mAb) or phorbol myristite acetate plus calcium ionophore in the presence or absence of human placental low molecular weight suppressor factor, followed by Western blotting. Transfer on nitrocellulose filters so as to allow the revelation of the phosphorylations was realized by means of a specific antiphosphotyrosin mAb. The final revelation was obtained by chemiluminescence. Similar experiments were performed on anti\u2010V\u03b2\u2010stimulated BALB\/c splenocytes, as well as cyproflaxin\u2010treated cells, which are hyper\u2010responsive in cell proliferation assays in the presence of the filtrate. In parallel, cells that were stimulated by a specific anti\u2010V\u03b2 and were rendered specifically anergic were studied by a specific anti\u2010V\u03b2 and were rendered specifically anergic were studied for other TcR expression using an FACS and both fluorescein isothiocyanate (FITC) and phycoerythrin (PE)\u2010labelled, related and unrelated anti\u2010V\u03b2 mAbs.<\/jats:p><jats:p>RESULTS: The phosphorylation of the \u03b6 chain homodimer quantitatively defective in filtrate\u2010treated, anti\u2010V\u03b26\u2010stimulated splenocytes as well as in Jurkatt cells. In parallel, cells from cyproflaxin\u2010treated Jurkatt cells were showing enhanced phosphorylation of all bands. The labelling of filtrate\u2010treated anti\u2010V\u03b26\u2010stimulated cells by an unrelated anti\u2010V\u03b2 (anti\u2010V\u03b28) showed double expression of V\u03b2 chains. The percentage of cells expressing this unrelated V\u03b2 (V\u03b2 8) was normal.<\/jats:p><jats:p>CONCLUSIONS: T cell anergy induced by a filtrate is linked to defective phosphorylation of the \u03b6\u2010chain homodimer. The abnormal percentage of cells expressing TcR after filtrate treatment might be due to adsorption by unstimulated cells of soluble TcR V\u03b2\u2010chain, possibly as a result of excess synthesis followed by membrane protease cleavage, allowing release in a soluble form of TcR V\u03b2\u2010chain nonspecifically captured by other cells.<\/jats:p>","DOI":"10.1111\/j.1600-0897.1997.tb00294.x","type":"journal-article","created":{"date-parts":[[2011,9,6]],"date-time":"2011-09-06T12:04:44Z","timestamp":1315310684000},"page":"168-175","source":"Crossref","is-referenced-by-count":20,"title":["Induction of Transient Murine T Cell Anergy by a Low Molecular Weight Compound Obtained from Supernatants of Human Placental Cultures Is Linked to Defective Phosphorylation of TCR CD3 Chain"],"prefix":"10.1111","volume":"38","author":[{"given":"Jean Luc","family":"Volum\u00e9nie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Mognetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delphine","family":"de Smedt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Menu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9rard","family":"Chaouat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,6]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"363","article-title":"Characteristics of the maternal lymphoid response in mice to paternal strain alloantigens induced by homologous pregnancy","volume":"31","author":"Baines MG","year":"1976","journal-title":"Immunology"},{"key":"e_1_2_1_3_2","first-page":"1405","article-title":"Prolonged survival of skin homografts in parous mice","volume":"25","author":"Breyere EJ","year":"1960","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_1_4_2","first-page":"89","article-title":"Demonstration, nature and properties of antibodies eluted from the placenta and directed against paternal antigens","volume":"21","author":"Voisin GA","year":"1974","journal-title":"J Reprod Fertil"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(80)90032-7"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196405000-00011"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1983.tb01089.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.146.3.899"},{"key":"e_1_2_1_9_2","unstructured":"ChandlerP. BenjaminD. SimpsonE.:Tolerance to H\u2010Y antigens. In4th International Congress of Immunology Paris 1980 Book of Abstracts M.Fougereau J.Dausset(eds). Abstract 04 08 05."},{"key":"e_1_2_1_10_2","first-page":"13","article-title":"Facilitation reaction (enhancing antibodies and suppressor cells) and rejection reaction (sensitized cells) from the mother to the paternal antigens of the conceptus","volume":"35","author":"Chaouat G.","year":"1979","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03023306"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1962.tb45355.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1984.tb00100.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.4049\/jimmunol.149.6.2203","article-title":"Human decidual leukocytes from early pregnancy contain high number of gamma\u2010delta+ cells and show selective down regulation of alloreactivity","volume":"149","author":"Mincheva\u2010Nilsson L.","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5236.630"},{"issue":"12","key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.4049\/jimmunol.144.8.3008","article-title":"Murine pregnancy decidua produces a unique immunosuppressive molecule related to transforming growth factor b\u20102","volume":"144","author":"Clark DA","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_1_17_2","first-page":"33","article-title":"Immunosuppressive properties of human placenta: Study of supernatants from short term syncytiotrophoblast cultures","volume":"9","author":"Culouscou JM","year":"1986","journal-title":"Cell Immunol"},{"key":"e_1_2_1_18_2","first-page":"133","volume-title":"Reproductive Immunology","author":"Hamaoka T.","year":"1983"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1987.tb00107.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0769-2625(84)81154-X"},{"key":"e_1_2_1_21_2","first-page":"341","article-title":"Immunoactive products of human placenta","volume":"119","author":"Menu E.","year":"1989","journal-title":"I. An Immunoregulatory factor obtained from expiant cultures of human placenta inhibits CTL generation and cytotoxic effector activity. Cell Immunol"},{"key":"e_1_2_1_22_2","first-page":"254","article-title":"Immunoactive products of human placenta","volume":"3","author":"Menu E.","year":"1991","journal-title":"III. Characterization of an inhibitor affecting lymphocyte proliferation. Reg Immunol"},{"key":"e_1_2_1_23_2","first-page":"195","article-title":"Immunoactive products of human placenta","volume":"20","author":"Menu E.","year":"1991","journal-title":"IV. Immunoregulatory factors obtained from cultures of human placenta inhibit local and general murine graft versus host reactions (GVHR). J Reprod Immunol"},{"key":"e_1_2_1_24_2","first-page":"197","volume-title":"Immunoactive products of human placenta. Biologie cellulaire et mol\u00e9culaire de la relation materno fetale","author":"Menu E.","year":"1991"},{"key":"e_1_2_1_25_2","first-page":"11","article-title":"Immunoactive products of placenta","volume":"36","author":"Djian V.","year":"1996","journal-title":"V) Immunoregulatory properties of a low molecular weight compound obtained from human placental cultures. Am J Reprod Immunol."},{"key":"e_1_2_1_26_2","volume-title":"Current Protocols Immunology Vol 1. Section 3.5","author":"Mage MG","year":"1994"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"5083","DOI":"10.4049\/jimmunol.155.11.5083","article-title":"Defective TcR chain phosphorylation in SEB induced anergic T cells","volume":"155","author":"Migita K.","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_1_28_2","first-page":"126","article-title":"Immunoactive products of placenta (I)","volume":"2","author":"Chaouat G.","year":"1980","journal-title":"J Reprod Immunol"},{"key":"e_1_2_1_29_2","doi-asserted-by":"crossref","first-page":"3497","DOI":"10.4049\/jimmunol.140.11.3947","article-title":"Specific defects in the anti listerial immune response in discrete regions of the murine uterus and placenta account for the susceptibility to infection","volume":"140","author":"Redline RW","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90235-D"},{"key":"e_1_2_1_31_2","doi-asserted-by":"crossref","first-page":"4562","DOI":"10.4049\/jimmunol.151.9.4562","article-title":"Synthesis of T Helper\u20102 cytokines at the maternal fetal interface","volume":"151","author":"Lin H.","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_1_32_2","first-page":"4261","article-title":"II\u201010 prevents inflammatory cytokine\u2010mediated fetal death and is inducible by tau interferon","volume":"154","author":"Chaouat G.","year":"1995","journal-title":"J Immunol"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0897.1997.tb00294.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0897.1997.tb00294.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T10:46:18Z","timestamp":1698403578000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0897.1997.tb00294.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["10.1111\/j.1600-0897.1997.tb00294.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0897.1997.tb00294.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}