{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:59:28Z","timestamp":1783576768456,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,12,14]],"date-time":"2004-12-14T00:00:00Z","timestamp":1102982400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>Problem:\u2002<\/jats:bold> To analyse percentage of total and memory CD27<jats:sup>+<\/jats:sup> B\u2010cells and other lymphocyte subpopulations in the peripheral blood (PB) and follicular fluid (FF) of infertile married couples.<\/jats:p><jats:p><jats:bold>Method of study:\u2002<\/jats:bold> Forty\u2010eight couples from <jats:italic>in vitro<\/jats:italic> fertilization\/embryo transfer (IVF\/ET) programme were divided into four groups: patients with previous unsuccessful fertilization (<jats:italic>n<\/jats:italic>\u2003=\u200313), ectopic pregnancy (<jats:italic>n<\/jats:italic>\u2003=\u20038), multiple (at least three) failed IVF\/ET (<jats:italic>n<\/jats:italic>\u2003=\u200318) and missed abortions (<jats:italic>n<\/jats:italic>\u2003=\u20039). Control group consisted of 15 married couples with healthy children.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> PB memory CD27<jats:sup>+<\/jats:sup> B\u2010cells were significantly decreased in all groups of infertile patients compared with controls. First group had increased memory B\u2010cells percentages compared with the second group. The differences in the percentages of PB memory B\u2010cells in third and fourth group compared with the first group were not statistically significant. FF memory B\u2010cells in the first and third group were significantly increased compared with second and fourth group. The percentage of total FF B\u2010cells in all groups were significantly decreased compared with their percentage in PB. Male partners of women from the first group had had significantly increased percentages of memory B\u2010cells compared with the partners of women from the second group. Percentage of total T\u2010 and B\u2010cells, CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cells, NK cells and activated HLA\u2010DR<jats:sup>+<\/jats:sup> T\u2010cells in all groups were not significantly different from controls. We found no statistically significant difference between immunoglobulin E levels in all groups of patients. We found lower levels of IgA and IgM in FF compared with serum in all groups.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Infertile patients have significantly decreased percentage of CD27<jats:sup>+<\/jats:sup> B\u2010cells in the PB. Abnormalities in the memory B\u2010cell compartment may contribute to the pathogenesis of infertility. In the T\u2010cell compartment abnormalities were not detected. It appears that hormonal stimulation did not influence cellular immunity parameters.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2004.00238.x","type":"journal-article","created":{"date-parts":[[2004,12,14]],"date-time":"2004-12-14T16:20:53Z","timestamp":1103041253000},"page":"379-385","source":"Crossref","is-referenced-by-count":18,"title":["B\u2010 and T\u2010cells in the Follicular Fluid and Peripheral Blood of Patients Undergoing IVF\/ET Procedures"],"prefix":"10.1111","volume":"52","author":[{"given":"Sanja","family":"Vujisi\u0107","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Snje\u017eana 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