{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:33:23Z","timestamp":1791077603923,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,8]],"date-time":"2005-02-08T00:00:00Z","timestamp":1107820800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:p><jats:bold>Problem:\u2002<\/jats:bold> Toll\u2010like receptors (TLRs) are expressed on placental cells. The aim of this study is to analyze signaling components activated in placenta cells after TLR ligand engagement.<\/jats:p><jats:p><jats:bold>Methods of study:\u2002<\/jats:bold> In chorioncarcimoma cell lines the regulation of TLRs was determined by real time polymerase chain reaction as well as by fluorescence\u2010activated cell sorter analysis. Activation of NF\u2010<jats:italic>\u03ba<\/jats:italic>B was determined in a reporter assay system and the activation of the mitogen\u2010activated protein kinase signaling pathways by immunoblot analysis.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Both lipopolysaccharide (LPS) and DNA oligonucleotides containing unmethylated CpG motifs (CpG) induced the enhanced expression of TLR2 mRNA as well as a TLR2 surface protein expression. Functionally, incubation of JAR cells with microbial stimuli such as LPS activated NF\u2010<jats:italic>\u03ba<\/jats:italic>B, as well as the phosphorylation of ERK1\/2 and p38 MAP kinases and secretion of interleukin\u20108.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The functional expression of TLRs on placental cells may play an important role in the initiation of an immune response in the developing fetus.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2004.00247.x","type":"journal-article","created":{"date-parts":[[2005,2,8]],"date-time":"2005-02-08T10:42:21Z","timestamp":1107859341000},"page":"77-84","source":"Crossref","is-referenced-by-count":39,"title":["Regulation and Signal Transduction of Toll\u2010Like Receptors in Human Chorioncarcinoma Cell Lines"],"prefix":"10.1111","volume":"53","author":[{"given":"Daniela","family":"Klaffenbach","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Rascher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"R\u00f6llinghoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"D\u00f6tsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udo","family":"Mei\u00dfner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Schnare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.083001.084359"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.4.615"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00796.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/44605"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/35047123"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35099560"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/32588"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00700-4"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1997.tb00511.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.2.554"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2002.01491.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011788"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200207)32:7<1958::AID-IMMU1958>3.0.CO;2-U"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni0901-835"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094351"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021890"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021787"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114265"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-1-122"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/smim.2000.0230"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.11.5998"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.11.5629"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80086-2"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02786486"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0897.2004.00247.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0897.2004.00247.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0897.2004.00247.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T01:30:35Z","timestamp":1696987835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0897.2004.00247.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["10.1111\/j.1600-0897.2004.00247.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0897.2004.00247.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,2]]}}}