{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:16:26Z","timestamp":1769062586253,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T00:00:00Z","timestamp":1109116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:p><jats:bold>Problem:\u2002<\/jats:bold> The aim of this study was to investigate the relationship between apoptosis by the mitochondrial pathway and luteal function in human granulosa cells.<\/jats:p><jats:p><jats:bold>Method of study:\u2002<\/jats:bold> Granulosa cells were obtained by ultrasound\u2010guided follicular aspiration from patients undergoing <jats:italic>in vitro<\/jats:italic> fertilization and embryo transfer. After the addition of RU486, cells were stained with a mitochondria\u2010specific fluorescent dye, MitoTracker Red CMxRos. Using flow cytometry and National Institute of Health image, the mitochondrial fluorescent area was measured. After staining with Hoechst 33258 dye, the number of apoptotic bodies per 1000 cells were counted at random on photomicrographs. Homogenates were used for sodium dodecyl sulphate\u2010polyacrylamide gel electrophoresis and Western blot analysis using antibodies against cytochrome <jats:italic>c<\/jats:italic> or caspase\u20103.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The incidence of apoptotic bodies increased and the mitochondrial membrane potential decreased time dependently. The opposite effect was observed dose dependently with RU486 treatment. Western blot analysis showed increased cytochrome <jats:italic>c<\/jats:italic> expression, after treatment with 1\u20132\u2003<jats:italic>\u03bc<\/jats:italic>g\/mL of RU486 which then decreased with 5\u201310\u2003<jats:italic>\u03bc<\/jats:italic>g\/mL of RU486. Caspase\u20103 expression increased dose dependently with RU486.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> These results suggest that the activation of caspase\u20103 caused by cytochrome <jats:italic>c<\/jats:italic> released from mitochondria plays an important role in apoptosis\u2010related luteal function in human granulosa cells.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2005.00258.x","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T08:59:09Z","timestamp":1109149149000},"page":"144-152","source":"Crossref","is-referenced-by-count":14,"title":["Role of Apoptosis Controlled by Cytochrome <i>c<\/i> Released from Mitochondria for Luteal Function in Human Granulosa 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