{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T17:53:32Z","timestamp":1721152412865},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,5]],"date-time":"2005-04-05T00:00:00Z","timestamp":1112659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p><jats:bold>Problem:\u2002<\/jats:bold>The upregulation of inflammatory substances threatens pregnancy. Interleukin\u201018 (IL\u201018) is elevated in women who miscarried. The purpose of this study was to develop a pig model of chorioamnionitis to study the effect of bacterial virulence on IL\u201018 response in experimentally infected amnion.<\/jats:p><jats:p><jats:bold>Method of study:\u2002<\/jats:bold>A total of 20,000 colony\u2010forming units of<jats:italic>Escherichia coli<\/jats:italic>(an enteropathogenic O55 strain, EPEC or O86 non\u2010pathogenic strain) were administered into the amniotic cavity of pig fetuses at 70% of gestation for 10\u2003hr. Fetal amniotic fluid samples were analyzed for IL\u201018 levels by enzyme\u2010linked immunosorbent assay. The expression of IL\u201018 was studied also by immunohistochemistry on cryostat sections through amniotic membranes and pathological changes were observed by electron microscopy.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>Both<jats:italic>E. coli<\/jats:italic>strains propagated in amniotic fluids and reached similar counts. Only EPEC, however, caused a significant increase of IL\u201018 amniotic fluid levels (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001) and cytokine expression in the amniotic epithelium.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>The levels of IL\u201018 in infected amniotic fluids correlated with bacterial virulence and pathological changes in the amnion.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2005.00273.x","type":"journal-article","created":{"date-parts":[[2005,4,5]],"date-time":"2005-04-05T10:35:46Z","timestamp":1112697346000},"page":"255-260","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Bacterial Virulence on IL\u201018 Expression in the Amnion Infected with<i>Escherichia coli<\/i>"],"prefix":"10.1111","volume":"53","author":[{"given":"Alla","family":"\u0160pl\u00edchalov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilja","family":"Trebichavsk\u00fd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Muneta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"\u0160pl\u00edchal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(91)90707-X"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.12.3560"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-5576(96)00042-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2001.d01-11.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4004(97)80045-8"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000010165"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.2.445"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(00)00064-4"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02815625"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(98)70142-5"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70347-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02471905"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1995.tb00932.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(98)70625-8"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/jmf.13.1.32.38"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/378088a0"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.10.3966-3972.1995"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-001-0307-1"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/374751"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108311200"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1078\/0171-2985-00052"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.1.294-297.2002"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02923528"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1177\/25.7.70450"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1089\/107999000312487"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(99)00166-0"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(02)00025-9"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2003.00045.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.Supplement_4.S394"},{"key":"e_1_2_6_31_2","first-page":"147","article-title":"Neonatal sepsis in hospital born babies","volume":"30","author":"Kaushik SL","year":"1998","journal-title":"J Commun Dis"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012657"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2001.0914"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.7.6.701"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh108"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016626620137"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02931560"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.25.239"},{"key":"e_1_2_6_39_2","doi-asserted-by":"crossref","first-page":"523","DOI":"10.33549\/physiolres.930241","article-title":"Escherichia coli administered into pig amniotic cavity appear in fetal airways and attract macrophages into fetal lungs","volume":"51","author":"Splichal I","year":"2002","journal-title":"Physiol Res"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(03)00339-0"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-0528.2003.02445.x"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2000.108881"},{"key":"e_1_2_6_43_2","first-page":"565","article-title":"Cytokines and chemokines in serum and urine as early predictors to identify septic patients on intensive care unit","volume":"12","author":"Kabir K","year":"2003","journal-title":"Int J Mol Med"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0897.2005.00273.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0897.2005.00273.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0897.2005.00273.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T16:08:11Z","timestamp":1706112491000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0897.2005.00273.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,5]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,5]]}},"alternative-id":["10.1111\/j.1600-0897.2005.00273.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0897.2005.00273.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4,5]]}}}