{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T04:45:20Z","timestamp":1744173920313},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T00:00:00Z","timestamp":1132272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Brucellosis causes abortion in domestic animals and Malta fever in humans. Comparison of <jats:italic>Brucella<\/jats:italic> species genomes may reveal potential virulence mechanisms. Engineering bioluminescent <jats:italic>Brucella<\/jats:italic> would permit monitoring bacterial dissemination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Microarray of the <jats:italic>B. melitensis<\/jats:italic> genome allowed comparison of gene content from six <jats:italic>Brucella<\/jats:italic> species. Bioluminescent <jats:italic>B. melitensis<\/jats:italic> strains were developed using transposon mutagenesis permitting the study of pathogenic <jats:italic>Brucella<\/jats:italic> in mice. Monitoring bacterial dissemination as well as organ localization permits evaluating the role of genes and genomic islands in mutant bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparative genomic analysis revealed 217 ORFs altered in five <jats:italic>Brucella<\/jats:italic> species and were often found in islands. Bioluminescent bacteria disseminated from the injection site to liver, spleen, inguinal lymph nodes, testes and submanibular region.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Genomic islands contribute to <jats:italic>Brucella<\/jats:italic> pathogenicity. Biophotonic imaging suggests that <jats:italic>Brucella<\/jats:italic> dissemination in mice parallels acute and chronic infections of humans.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2005.00329.x","type":"journal-article","created":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T23:16:37Z","timestamp":1132355797000},"page":"358-368","source":"Crossref","is-referenced-by-count":16,"title":["Unraveling <i>Brucella<\/i> Genomics and Pathogenesis in Immunocompromised IRF\u20101<sup>\u2212\/\u2212<\/sup> Mice"],"prefix":"10.1111","volume":"54","author":[{"given":"Gireesh","family":"Rajashekara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Krepps","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Eskra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Mathison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison","family":"Montgomery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Splitter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970201"},{"key":"e_1_2_6_3_2","first-page":"3","article-title":"Effect of the primary distribution of the microbial aerosol in the respiratory system on the size of the infecting dose","volume":"50","author":"Gapochko KG","year":"1973","journal-title":"Zh Mikrobiol Epidemiol Immunobiol"},{"key":"e_1_2_6_4_2","volume-title":"Brucellosis in Nonhuman Primates following Aerosol Exposure to Brucella melitensis Strain 16M","author":"Borschel R","year":"1998"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.8.2035-2036.1994"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0195-6701(89)90136-9"},{"key":"e_1_2_6_7_2","volume-title":"Brucellosis Surveillance. 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