{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T17:09:07Z","timestamp":1697044147851},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,3,30]],"date-time":"2006-03-30T00:00:00Z","timestamp":1143676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>In spite of their key immunological role, <jats:italic>\u03b1<\/jats:italic><jats:italic>\u03b2<\/jats:italic><jats:sup>+<\/jats:sup> T cells residing in endometrium have not been extensively explored. We analyzed here expression profile of <jats:italic>TCRBV<\/jats:italic> genes in normal human endometrium compared with peripheral blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples were taken from normal reproductive women. RT\u2010PCR using <jats:italic>BV<\/jats:italic>\u2010gene specific primers was performed on blood and endometrial samples. After blotting, hybridization with radiolabelled probe and autoradiography, relative expression of each <jats:italic>TCRBV<\/jats:italic> family was determined. Clonal expansions of the over\u2010expressed genes were assessed by CDR3 length polymorphism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Only one gene (<jats:italic>TCRBV7<\/jats:italic>) was expressed significantly and two other genes marginally more in the endometrium compared with blood. All three <jats:italic>TCRBV<\/jats:italic> genes examined showed a rather restricted pattern in the endometrium in contrast to polyclonal patterns in the blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results stress the similarities between T cells residing in different mucosal tissues and provide a basis for future investigations about endometrial T cells and their antigen specificities in gynecological problems.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2006.00375.x","type":"journal-article","created":{"date-parts":[[2006,3,30]],"date-time":"2006-03-30T15:27:27Z","timestamp":1143732447000},"page":"349-359","source":"Crossref","is-referenced-by-count":4,"title":["Expression Profile and Clonality of T\u2010Cell Receptor Beta Variable Genes in Normal Human 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