{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T01:10:34Z","timestamp":1696986634441},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T00:00:00Z","timestamp":1146614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>11\u03b2\u2010Hydroxysteroid dehydrogenase (11\u03b2\u2010HSD) plays an important role in regulating active glucocorticoid reaching the fetus. In normal pregnancy, placental 11\u03b2\u2010HSD functions primarily in oxidative direction. Placental tissue of patients with pregnancies complicated by pre\u2010eclampsia exhibit significantly lower type 1 and 2 11\u03b2\u2010HSD activities and significantly high cortisol level in cord blood suggesting fetal exposure to higher level of active glucocorticoids. The activity of 11\u03b2\u2010HSD in gestational trophoblastic disease has not been determined. The objective of this study was to assess 11\u03b2\u2010HSD activity in tissue from normal second trimester and pregnancies complicated by hydatidiform mole.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Normal placental tissues were obtained from patients undergoing termination of pregnancy, and from patients undergoing uterine evacuation for hydatidiform mole. Both nicotinamide adenine dinucleotide (NAD)\u2010 and nicotinamide adenine dinucleotide phosphate (NADP)\u2010dependent activities were assayed in central villous tissue. Comparison of groups was performed using Student's <jats:italic>t<\/jats:italic>\u2010test. A <jats:italic>P<\/jats:italic>\u2010value of 0.05 was considered significant. Data are presented as mean\u2003\u00b1\u2003S.D.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Tissue obtained from five patients with pathology\u2010proven hydatidiform mole demonstrated significantly lower 11\u03b2\u2010HSD activities compared with placental tissue obtained from normal pregnancies. The mean NAD\u2010dependent 11\u03b2\u2010HSD activity in normal placentas was 386\u2003\u00b1\u2003109\u2003pmol\/min\/g placenta and in hydatidiform mole was 74\u2003\u00b1\u200354\u2003pmol\/min\/g placenta (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01). The mean NADP\u2010dependent 11\u03b2\u2010HSD activity in normal placentas was 370\u2003\u00b1\u2003120\u2003pmol\/min\/g placenta and in trophoblastic disease was 68\u2003\u00b1\u200369\u2003pmol\/min\/g placenta (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data indicate significant impairment in the ability of hydatidiform mole tissue to inactivate glucocorticoids.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2006.00382.x","type":"journal-article","created":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T00:49:06Z","timestamp":1146703746000},"page":"415-419","source":"Crossref","is-referenced-by-count":2,"title":["11\u03b2\u2010Hydroxysteroid Dehydrogenase Activity in Pregnancies Complicated by Hydatidiform 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