{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T10:30:49Z","timestamp":1781087449014,"version":"3.54.1"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,3,13]],"date-time":"2007-03-13T00:00:00Z","timestamp":1173744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>To investigate possible roles of the natural killer (NK) cell receptor killer immunoglobulin\u2010like receptor (KIR)2DL4 expressed on uterine NK (uNK) cells during pregnancy, we investigated KIR2DL4 expression on uNK cells isolated from patients with early recurrent spontaneous abortion (RSA) and normal early pregnancy women, and functions of KIR2DL4 was analyzed <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods of the study<\/jats:title><jats:p>Semi\u2010quantitative RT\u2010PCR analysis was introduced to detect KIR2DL4 messenger RNA (mRNA) expression on uNK cells. Cytotoxicity and cytokine production as the result of interaction of KIR2DL4 and its ligand human leukocyte antigen (HLA)\u2010G were analyzed <jats:italic>in vitro<\/jats:italic> with lactic dehydrogenase releasing method and enzyme\u2010linked immunosorbent assay, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No significant difference in KIR2DL4 mRNA expression was observed, while the KIR2DL4 protein level in isolated uNK cells is much higher in normal controls than that in RSA patients. Data showed that HLA\u2010G transfection could not reverse the lysis of uNK against HLA\u2010G transfected K562 cells but induced cytokine production. Furthermore, we demonstrated that, via KIR2DL4, membrane\u2010bound HLA\u2010G could induce high cytotoxicity and cytokine production in a high cytotoxic, IL\u20102 dependent human NK cell line NK\u201092 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggest that KIR2DL4 might play a crucial implication for human pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2007.00469.x","type":"journal-article","created":{"date-parts":[[2007,3,13]],"date-time":"2007-03-13T07:11:12Z","timestamp":1173769872000},"page":"233-242","source":"Crossref","is-referenced-by-count":50,"title":["Possible Roles of KIR2DL4 Expression on uNK Cells in Human 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