{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:17:37Z","timestamp":1791199057102,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,10,24]],"date-time":"2008-10-24T00:00:00Z","timestamp":1224806400000},"content-version":"vor","delay-in-days":266,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:p><jats:bold>Problem\u2002<\/jats:bold>Endometritis after insemination is ubiquitous in the horse and is associated with semen and\/or bacteria in the uterus. In up to 40% of horses, inflammation persists causing infertility. An endometrial explant culture was developed to study uterine secretion of prostaglandin F<jats:sub>2\u03b1<\/jats:sub>(PGF<jats:sub>2\u03b1<\/jats:sub>) in response to physiological and pathological challenge.<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold>Uteri were collected from mares, the endometrium dissected and explants from the uterine body or horn cultured in William\u2019s or RPMI medium. The response of explants to oxytocin, semen or bacteria compared to untreated tissue was tested by collecting medium after 24 and 72\u2003hr and measuring PGF<jats:sub>2\u03b1<\/jats:sub>by radioimmunoassay.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold>Explants from the uterine horn and cultured in William\u2019s medium secreted the most PGF<jats:sub>2\u03b1<\/jats:sub>after challenge with oxytocin. Explants treated with semen produced a PGF<jats:sub>2\u03b1<\/jats:sub>response after 72\u2003hr. Explants collected from mares in the transition season treated with killed<jats:italic>S. zooepidemicus<\/jats:italic>or<jats:italic>E. coli<\/jats:italic>lipopolysaccharide (LPS) secreted increased concentrations of PGF<jats:sub>2\u03b1<\/jats:sub>after 24 and 72\u2003hr. The response to LPS was inhibited by polymyxin B. Follicular and luteal phase explants did not respond to treatments.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold>An endometrial explant culture was developed that measured PGF<jats:sub>2\u03b1<\/jats:sub>and may be used to study endometritis.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2007.00548.x","type":"journal-article","created":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T13:13:03Z","timestamp":1282137183000},"page":"105-117","source":"Crossref","is-referenced-by-count":29,"title":["ORIGINAL ARTICLE: Endometrial Explant Culture for Characterizing Equine 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