{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:36:04Z","timestamp":1792740964389,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,1,13]],"date-time":"2008-01-13T00:00:00Z","timestamp":1200182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>To better understand innate immune responses to sexually\u2010transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll\u2010like receptor (TLR) expression from a population of women is needed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>TLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TLR1\u20133 and 5\u20139 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7\u20139 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2007.00566.x","type":"journal-article","created":{"date-parts":[[2008,2,11]],"date-time":"2008-02-11T06:25:15Z","timestamp":1202711115000},"page":"212-224","source":"Crossref","is-referenced-by-count":127,"title":["ORIGINAL ARTICLE: Quantification and Comparison of Toll\u2010Like Receptor Expression and Responsiveness in Primary and Immortalized Human Female Lower Genital Tract Epithelia"],"prefix":"10.1111","volume":"59","author":[{"given":"Melissa M.","family":"Herbst\u2010Kralovetz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison 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