{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T22:59:38Z","timestamp":1789340378891,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p><jats:bold>Problem\u2002<\/jats:bold> Determination of subpopulations of T lymphocytes, natural killer (NK) and activation status, in peripheral blood during the mid\u2010luteal phase from patients with unexplained recurrent spontaneous abortion (RSA).<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold> Peripheral blood samples from non\u2010pregnant women with RSA and normal multiparous were taken and evaluated for subpopulations of T lymphocytes: CD4, CD8, (\u2018naive\u2010like\u2019 and \u2018memory\u2010like\u2019), TCR receptor (\u03b1\u03b2 and \u03b3\u03b4), activation status by CD69<jats:sup>+surface or intracellular<\/jats:sup>\/CD3<jats:sup>+<\/jats:sup>, and NK cells (CD16<jats:sup>+<\/jats:sup>\/CD56<jats:sup>dim<\/jats:sup>\/CD3<jats:sup>\u2212<\/jats:sup>, CD16<jats:sup>+<\/jats:sup>\/CD56 <jats:sup>bright<\/jats:sup>\/CD3<jats:sup>\u2212<\/jats:sup>, CD69<jats:sup>+surface or intracellular<\/jats:sup>\/CD56<jats:sup>+<\/jats:sup>\/CD3<jats:sup>\u2212<\/jats:sup> cells).<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> The evaluation of T lymphocytes only showed an increase in the expression of CD69 (surface and intracellular) in the RSA group. Additionally, we observed an increase in the total NK cells, CD56<jats:sup>+<\/jats:sup> NK cells percentages, CD56<jats:sup>dim<\/jats:sup> NK cells and CD69 NK cells in RSA group.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> These observations support the concept that immunological activation of T lymphocytes and NK cells could be involved in peripheral blood during the mid\u2010luteal phase in patients with unexplained RSA.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2008.00591.x","type":"journal-article","created":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T07:17:10Z","timestamp":1213341430000},"page":"66-74","source":"Crossref","is-referenced-by-count":22,"title":["ORIGINAL ARTICLE: Immunophenotype of Peripheral T Lymphocytes, NK Cells and Expression of CD69 Activation Marker in Patients with Recurrent Spontaneous Abortions, During the Mid\u2010Luteal 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