{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T23:49:52Z","timestamp":1768002592212,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T00:00:00Z","timestamp":1228953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p><jats:bold>Problem\u2002<\/jats:bold> Homing of lymphocytes and NK cells into the decidua and its regulation has been very controversially discussed. Therefore, we aimed to establish an <jats:italic>in vivo<\/jats:italic> simulation method for analysis of homing behavior, which might be also useful for other cells such as stem or tumor cells.<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold> A human term placenta has been perfused with medium to elute blood and then with maternal autologous carboxyfluorescein succinimidyl ester (CFSE)\u2010labeled peripheral blood lymphocytes for 3\u2003hr and rinsed for another 2\u2003hr. Tissue was analysed histologically for detection of labeled cells. Labeled lymphocytes and beads in perfusate have been identified and counted by flow cytometry.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> At the moment of tissue fixation for histology, the perfusate was free of labeled cells. Labeled perfused lymphocytes have been found adhered and integrated in vessel wall structures, in decidual stroma and as colonies in individual villi.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> Placenta perfusion with a lymphocyte suspension is feasible without plugging the tube system. Time is sufficient for cells to adhere and to migrate into the stroma. Also some villi have been infiltrated which might be caused by inflammatory stimuli. The perfusion system might be useful to test substances for their capacity to influence homing of lymphocytes or other cells.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2008.00666.x","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T18:36:58Z","timestamp":1229020618000},"page":"19-25","source":"Crossref","is-referenced-by-count":7,"title":["SHORT COMMUNICATION: Development of a Human Model to Study Homing Behavior of Immune Cells into Decidua and Placental Villi Under <i>Ex Vivo<\/i> Conditions"],"prefix":"10.1111","volume":"61","author":[{"given":"Joana","family":"Heinzelmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uta","family":"Enke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Seyfarth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekkehard","family":"Schleussner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoine","family":"Malek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udo R.","family":"Markert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137430"},{"key":"e_1_2_6_3_2","first-page":"129","article-title":"Cell populations in human early pregnancy decidua: characterization and isolation of large granular lymphocytes by flow cytometry","volume":"65","author":"Starkey PM","year":"1988","journal-title":"Immunology"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2005.03.004"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2008.00598.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-08-105965"},{"key":"e_1_2_6_7_2","first-page":"55","article-title":"Osteopontin expression in human decidua is associated with decidual natural killer cells recruitment and regulated by progesterone","volume":"22","author":"Qu X","year":"2008","journal-title":"In Vivo"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.4.2367"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei436"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000087952"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00267-4"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0202-157"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.9.1105"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(02)00418-0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1671"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2006.00420.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.1999.00871.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2005.05.009"},{"key":"e_1_2_6_19_2","first-page":"458","article-title":"The perfusion of the placenta in vitro used in the study of the biosynthesis of estrogens","volume":"155","author":"Cedard L","year":"1961","journal-title":"C R Seances Soc Biol Fil"},{"key":"e_1_2_6_20_2","first-page":"369","article-title":"Influence of drugs on vasoactive peptides and amines in perfused human placenta","volume":"44","author":"Klinge E","year":"1966","journal-title":"Ann Med Exp Biol Fenn"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0002-9378(16)34635-X","article-title":"Extracorporeal perfusion of the human placenta. 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