{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T07:56:28Z","timestamp":1771487788092,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,11]],"date-time":"2009-01-11T00:00:00Z","timestamp":1231632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:p><jats:bold>Problem\u2002<\/jats:bold> Comparison of two types of immunocompromised mouse strains (SCID and NOD\/SCID) for production of human antisperm antibodies (AsA).<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold> Human peripheral blood lymphocytes (PBL) were grafted to mouse peritoneal cavity and sensitized with natively glycosylated and N\u2010deglycosylated sperm extracts.<\/jats:p><jats:p><jats:bold>Results and conclusion\u2002<\/jats:bold> NOD\/SCID mice inoculated with hu\u2010PBLs exhibited higher AsA titres with a tendency for greater sperm agglutination than human AsA raised in SCID mouse model. A comparison between \u2018native\u2019 and deglycosylated sperm extracts revealed higher agglutination titres by sera induced with the latter ones. Inhibitory effect of human polyclonal AsA in sperm penetration assay, however, produced opposite results to that for agglutination. Western immunoblotting was used to evaluate reactive sperm antigens prior to and after <jats:italic>in situ<\/jats:italic> sensitization showing multiple bands different from positive reactions brought by original sera of <jats:italic>in vivo<\/jats:italic> primed AsA\u2010positive males. It seems that <jats:italic>in situ<\/jats:italic> generated AsA recognized sperm entities different from those revealed by blood donor\u2019s sera samples.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2008.00675.x","type":"journal-article","created":{"date-parts":[[2009,1,12]],"date-time":"2009-01-12T05:02:56Z","timestamp":1231736576000},"page":"147-157","source":"Crossref","is-referenced-by-count":3,"title":["ORIGINAL ARTICLE: <i>In situ<\/i> Reconstruction of Humoral Immune Response Against Sperm: Comparison of SCID and NOD\/SCID Mouse Models"],"prefix":"10.1111","volume":"61","author":[{"given":"Beata","family":"Grygielska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marzena","family":"Kamieniczna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Wiland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Kurpisz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,11]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-007-0075-2"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(91)90041-N"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00302-1"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg207"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gam026"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(95)00080-O"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80046-8"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200832"},{"key":"e_1_2_7_10_2","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.4049\/jimmunol.148.4.1065","article-title":"Human peripheral blood lymphocytes transplanted into SCID mice constitute an in vivo culture system exhibiting several parameters found in a normal humoral immune response and are a source of immunocytes for the production of human monoclonal antibodies","volume":"148","author":"Carlsson R","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_7_11_2","first-page":"163","article-title":"The in vivo production of specific human antibodies by vaccination of human\u2010PBL\u2010SCID mice","volume":"83","author":"Walker W","year":"1994","journal-title":"Immunology"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2004.00606.x"},{"key":"e_1_2_7_13_2","first-page":"35140","article-title":"Human immunoglobulin production in immunodeficient mice: enhancement by immunosuppression of host and in vitro activation of human mononuclear cells","volume":"90","author":"Cavacini LA","year":"1992","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_7_14_2","first-page":"369","article-title":"Human immunoglobulin production in Scid mice following primary sensitisation of human lymphocytes in situ","volume":"42","author":"Kurpisz M","year":"1994","journal-title":"Arch Immunol Ther Ex"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/301527a0"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.160166"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.1.180"},{"key":"e_1_2_7_18_2","first-page":"15.9.1","article-title":"The NOD mouse: a model for insulin\u2010dependent diabetes mellitus","volume":"4","author":"Leiter EH","year":"1997","journal-title":"Curr Protoc Immunol"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260314"},{"key":"e_1_2_7_20_2","first-page":"250","article-title":"Mouse monoclonal antibodies against human sperm: evidence for immunodominant glycosylated antigenic sites","volume":"78","author":"Kurpisz M","year":"1989","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1159\/000131876"},{"key":"e_1_2_7_23_2","first-page":"394","article-title":"Infertility status of male individuals with abnormal spermiogram evaluated by cytogenetic analysis and in vitro penetration assay","volume":"8","author":"Wiland E","year":"2002","journal-title":"Med Sci Monit"},{"key":"e_1_2_7_24_2","volume-title":"Laboratory Manual for the Examination of Human Semen and Sperm\u2010Cervical Mucus Interaction","author":"World Health Organization","year":"1999"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)55262-5"},{"key":"e_1_2_7_26_2","first-page":"86","volume-title":"Methods in Mammalian Embryology","author":"Biggers JD","year":"1971"},{"key":"e_1_2_7_27_2","first-page":"18.47","volume-title":"Molecular Cloning. 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