{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:29:22Z","timestamp":1783466962486,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,2,12]],"date-time":"2009-02-12T00:00:00Z","timestamp":1234396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:p><jats:bold>Problem\u2002<\/jats:bold> Among class Ib human leukocyte antigen (HLA) molecules, HLA\u2010E is known to be a major ligand of CD94\/NKG2 receptor on natural killer (NK) cells, and to play a pivotal role in recognition of extravillous trophoblasts (EVTs) by maternal immune cells. However, it is scarcely known how HLA\u2010E expression is regulated in EVTs.<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold> In this study, we investigated whether progesterone, an essential hormone in maintaining pregnancy, regulated HLA\u2010E expression in EVT\u2010like cell line, JEG\u20103. HLA\u2010E mRNA amount in cultured JEG\u20103 cells was assessed by real\u2010time PCR and cell\u2010surface HLA\u2010E protein was analyzed by flowcytometry.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Real\u2010time PCR showed 3.5\u2010fold increase 1\u2003hour after the addition of 1000\u2003ng\/ml progesterone. This response was dimished by the addition of RU486, an antagonist for progesterone receptor. Flowcytometry indicated that 1000\u2003ng\/ml progesterone slightly enhanced HLA\u2010E expression on the surface of JEG\u20103.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> These results suggest that progesterone up\u2010regulates HLA\u2010E expression in JEG\u20103 cells through the pathway mediated by progesterone receptor. Our findings might give a new insight into immunomodulatory function of progesterone at fetomaternal interface.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2008.00684.x","type":"journal-article","created":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T17:19:03Z","timestamp":1282151943000},"page":"221-226","source":"Crossref","is-referenced-by-count":9,"title":["ORIGINAL ARTICLE: Effect of Progesterone on HLA\u2010E Gene Expression in JEG\u20103 Choriocarcinoma Cell Line"],"prefix":"10.1111","volume":"61","author":[{"given":"Zhongying","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironobu","family":"Hyodo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Nagamatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Kawana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiharu","family":"Yasugi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Kozuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Taketani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,12]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/plac.1999.0496"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200006)30:6<1623::AID-IMMU1623>3.0.CO;2-M"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.3.1376"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"897","DOI":"10.4049\/jimmunol.141.3.897","article-title":"HLA\u2010E. 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