{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T15:45:47Z","timestamp":1747064747044},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,12,15]],"date-time":"2009-12-15T00:00:00Z","timestamp":1260835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:p> <jats:bold>Citation<\/jats:bold> Elito J Jr, Daher S, Fernandes da Silva MO, Marconi NMH, Pendeloski KPT, Moron AF, Camano L. Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy. Am J Reprod Immunol 2010; 63: 120\u2013125<\/jats:p><jats:p><jats:bold>Problem\u2002<\/jats:bold> In ectopic pregnancy, increased levels of vascular endothelial growth factor are present. The aims of this study were to determine the association between \u2212634C\/G, \u2212460T\/C, and +936C\/T vascular endothelial growth factor (VEGF) polymorphisms and ectopic pregnancy, and to determine whether serum levels of VEGF were affected by genetic factors.<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold> This is a case\u2013control study wherein 74 women with a history of ectopic pregnancy in a tertiary care center were compared to 134 post\u2010menopausal controls with two pregnancies and no ectopic pregnancy for the genotyping of VEGF polymorphisms. For 35 patients with the diagnosis of ectopic pregnancy, serum concentrations of VEGF were obtained before the treatment. Genotyping of VEGF (\u2212634C\/G, \u2212460T\/C, and +936C\/T) polymorphisms was performed by PCR, followed by endonuclease digestion. ELISA was performed to evaluate the VEGF serum levels.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> The \u2212634C\/G, \u2212460T\/C, and +936C\/T VEGF polymorphisms were not associated with ectopic pregnancy (<jats:italic>P<\/jats:italic>\u2003=\u20030.170, <jats:italic>P<\/jats:italic>\u2003=\u20030.285, and <jats:italic>P<\/jats:italic>\u2003=\u20030.700, respectively). The serum levels of VEGF were not associated with the genotype of \u2212634C\/G, \u2212460T\/C, and +936C\/T VEGF polymorphisms (<jats:italic>P<\/jats:italic>\u2003=\u20030.702; <jats:italic>P<\/jats:italic>\u2003=\u20030.347, and <jats:italic>P<\/jats:italic>\u2003=\u20030.256, respectively).<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> There was no association between ectopic pregnancy and \u2212634C\/G, \u2212460T\/C, and +936C\/T VEGF polymorphisms. 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