{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:15:17Z","timestamp":1767842117527,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,6,20]],"date-time":"2011-06-20T00:00:00Z","timestamp":1308528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p><jats:bold>Citation<\/jats:bold> Iwasawa Y, Kawana K, Fujii T, Schust DJ, Nagamatsu T, Kawana Y, Sayama S, Miura S, Matsumoto J, Adachi K, Hyodo H, Yamashita T, Kozuma S, Taketani Y. A possible coagulation\u2010independent mechanism for pregnancy loss involving \u03b2<jats:sub>2<\/jats:sub>glycoprotein 1\u2010dependent antiphospholipid antibodies and CD1d. Am J Reprod Immunol 2012; 67: 54\u201365<\/jats:p><jats:p><jats:bold>Problem\u2002<\/jats:bold> \u03b2<jats:sub>2<\/jats:sub>glycoprotein1 (\u03b2<jats:sub>2<\/jats:sub>GP1)\u2010dependent antiphospholipid antibodies (aPL) increase the risk for recurrent pregnancy loss. We address whether anti\u2010\u03b2<jats:sub>2<\/jats:sub>GP1 antibodies can interact with phosphatidylserine (PS)\u2010bearing CD1d on trophoblast cells and induce local inflammation.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> CD1d\u2010bearing choriocarcinoma cells were used in flow cytometry and immunoprecipitation experiments. CD1d\u2010mediated cytokine induction was assessed using antibody cross\u2010linking. Cytokine production during co\u2010culture of decidual lymphocytes with CD1d\u2010bearing cells was also examined.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Trophoblast surface\u2010expressed CD1d forms a complex with PS\u2010bound \u03b2<jats:sub>2<\/jats:sub>GP1. Anti\u2010\u03b2<jats:sub>2<\/jats:sub>GP1 mAb cross\u2010linking causes IL12p70 release from CD1d\u2010bearing cells. IL12p70 release from CD1d\u2010bearing trophoblast cells was also induced during co\u2010culture with human decidual lymphocytes. The addition of anti\u2010\u03b22GP1 mAb to co\u2010cultures resulted in a three\u2010fold increase in IL12p70 secretion. IFN\u03b3 secretion from decidual lymphocytes was also induced during co\u2010culture with anti\u2010\u03b22GP1 mAbs.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> \u03b2<jats:sub>2<\/jats:sub>GP1\u2010dependent IL12 release from CD1d\u2010bearing trophoblast in the presence of aPL may link the antiphospholipid syndrome to pregnancy loss via an inflammatory mechanism.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2011.01028.x","type":"journal-article","created":{"date-parts":[[2011,6,20]],"date-time":"2011-06-20T09:43:02Z","timestamp":1308562982000},"page":"54-65","source":"Crossref","is-referenced-by-count":7,"title":["A Possible Coagulation\u2010Independent Mechanism for Pregnancy Loss Involving \u03b2<sub>2<\/sub>glycoprotein 1\u2010Dependent Antiphospholipid Antibodies and 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