{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:54:23Z","timestamp":1778666063620,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T00:00:00Z","timestamp":1309737600000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:sec><jats:label\/><jats:p> <jats:bold>Citation<\/jats:bold> Morales\u2010Prieto DM, Schleussner E, Markert UR. Reduction in miR\u2010141 is Induced by Leukemia Inhibitory Factor and Inhibits Proliferation in Choriocarcinoma Cell line JEG\u20103. Am J Reprod Immunol 2011; 66 (Suppl. 1): 57\u201362<\/jats:p><\/jats:sec><jats:sec><jats:title>Problem<\/jats:title><jats:p>Starting from the peri\u2010implantation period, leukemia inhibitory factor (LIF) is a major regulator of trophoblast functions. Micro\u2010RNAs (miRNA) are short non\u2010coding RNA sequences, which regulate expression of genes at post\u2010transcriptional level. The influence of LIF on miRNA expression in trophoblastic cells has not yet been analyzed and was focus of this investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>JEG\u20103 choriocarcinoma cells have been stimulated with LIF for 1, 2, 4, 6, and 24\u2003hr. The expression of miR\u20109, miR\u2010141, miR\u201021, miR\u201093, and let\u20107g has been analyzed by real\u2010time PCR. Subsequently, miR\u2010141 has been silenced and over\u2010expressed to test its role in the proliferation of JEG\u20103 cells after 24 and 48\u2003hr.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u2010141 has been significantly downregulated by more than 50% after LIF stimulation, while miR\u201021 and miR\u201093 expression has been significantly upregulated. Silencing of miR\u2010141 completely inhibited the proliferation of JEG\u20103 cells, while over\u2010expression had no effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LIF regulates expression of miRNA in trophoblastic cells, which may be responsible for several functional effects induced by LIF.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2011.01037.x","type":"journal-article","created":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T17:44:17Z","timestamp":1309801457000},"page":"57-62","source":"Crossref","is-referenced-by-count":38,"title":["Reduction in miR\u2010141 is Induced by Leukemia Inhibitory Factor and Inhibits Proliferation in Choriocarcinoma Cell Line JEG\u20103"],"prefix":"10.1111","volume":"66","author":[{"given":"Diana 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