{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:18:49Z","timestamp":1789654729108,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,7,13]],"date-time":"2011-07-13T00:00:00Z","timestamp":1310515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:bold>Citation<\/jats:bold> Zaga\u2010Clavellina V, Martha RV\u2010M, Flores\u2010Espinosa P. <jats:italic>In vitro<\/jats:italic> secretion profile of pro\u2010inflammatory cytokines IL\u20101\u03b2, TNF\u2010\u03b1, IL\u20106, and of human beta\u2010defensins (HBD)\u20101, HBD\u20102, and HBD\u20103 from human chorioamniotic membranes after selective stimulation with <jats:italic>Gardnerella vaginalis.<\/jats:italic> Am J Reprod Immunol 2012; 67: 34\u201343<\/jats:p><\/jats:sec><jats:sec><jats:title>Problem<\/jats:title><jats:p>Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with <jats:italic>Gardnerella vaginalis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Using a two\u2010compartment experimental model, 1\u2003\u00d7\u200310<jats:sup>6<\/jats:sup>\u2003CFU\/mL of <jats:italic>G.\u00a0vaginalis<\/jats:italic> were added to either the amnion or choriodecidua face or to both. Concentrations of IL\u20101\u03b2, TNF\u2010\u03b1, and IL\u20106, as well as human beta defensins (HBD) 1\u20103 were quantified by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In comparison with control conditions and regardless of the stimulation modality, IL\u20101\u03b2 and IL\u20106 increased 4\u2010fold and 28\u2010fold, respectively, in the choriodecidual compartment. HBD\u20101 increased 2\u2010fold mainly in the amniotic compartment when the stimulus was applied directly to this region. HBD\u20102 and HBD\u20103 increased an average of 2\u2010 and 8\u2010fold, respectively, in the choriodecidual region.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Stimulation with <jats:italic>G.\u00a0vaginalis<\/jats:italic> induced a tissue\u2010specific secretion profile of 1L\u20101\u03b2, IL\u20106, and HBD 1\u20103 in the chorioamniotic membranes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2011.01054.x","type":"journal-article","created":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T03:07:52Z","timestamp":1310612872000},"page":"34-43","source":"Crossref","is-referenced-by-count":38,"title":["<i>In Vitro<\/i> Secretion Profile of Pro\u2010Inflammatory Cytokines IL\u20101\u03b2, TNF\u2010\u03b1, IL\u20106, and of Human Beta\u2010Defensins (HBD)\u20101, HBD\u20102, and HBD\u20103 from Human Chorioamniotic Membranes After Selective Stimulation with <i>Gardnerella 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