{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:24:25Z","timestamp":1761863065138},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,9,14]],"date-time":"2011-09-14T00:00:00Z","timestamp":1315958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p><jats:bold>Citation<\/jats:bold> Alvero AB, Montagna MK, Craveiro V, Liu L, Mor G. Distinct subpopulations of epithelial ovarian cancer cells can differentially induce macrophages and T regulatory cells toward a pro\u2010tumor phenotype. Am J Reprod Immunol 2012; 67: 256\u2013265<\/jats:p><jats:p><jats:bold>Problem\u2002<\/jats:bold> Presence of immune infiltrates in the tumor does not always correlate with an anti\u2010tumoral immune response. We previously identified two subpopulations of epithelial ovarian cancer (EOC) cells with differential cytokine profile. We hypothesize that these two subpopulations of EOC cells may differentially regulate the immune phenotype in the tumor microenvironment and therefore affect the immune response.<\/jats:p><jats:p><jats:bold>Method of Study\u2002<\/jats:bold> Macrophages derived from CD14+ monocytes and naive CD4+T cells were treated with conditioned media from two subpopulations of EOC cells. Differentiation markers and phagocytic activity were measured by western blot analysis and flow cytometry. Cytokine levels were quantified using xMAP technology.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Type I EOC cells are able to enhance macrophages\u2019 capacity for tumor repair and renewal by enhancing expression of scavenger receptors and by promoting the secretion of cytokines associated with tissue repair. On the other hand, type II EOC cells are able to create a tolerant microenvironment and prevent an immune response by inducing macrophages\u2019 to secrete IL\u201010 and by promoting the generation of T regs.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> We demonstrate that each ovarian cancer cell subpopulation can induce a unique phenotype of macrophages and T cells, both associated with tumor\u2010supportive function.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2011.01068.x","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T08:20:35Z","timestamp":1316074835000},"page":"256-265","source":"Crossref","is-referenced-by-count":38,"title":["Distinct Subpopulations of Epithelial Ovarian Cancer Cells Can Differentially Induce Macrophages and T Regulatory Cells Toward a Pro\u2010Tumor Phenotype"],"prefix":"10.1111","volume":"67","author":[{"given":"Ayesha B.","family":"Alvero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele K.","family":"Montagna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinicius","family":"Craveiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanzhen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gil","family":"Mor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.10.2517"},{"key":"e_1_2_6_3_2","first-page":"766","article-title":"Ovarian cancer. Clinical practice guidelines in oncology","volume":"6","author":"Morgan RJ","year":"2008","journal-title":"J Natl Compr Canc Netw"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-1167"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.11.8.14967"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2006.00441.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.1.7533"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.112"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000028096"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0602325"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6166"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.10.001"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(02)02302-5"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710686105"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(98)00143-5"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2009.02369.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2010.00968.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2004.00156.x"},{"key":"e_1_2_6_19_2","first-page":"5573","article-title":"Epithelial ovarian cancer secrete functional Fas Ligand","volume":"63","author":"Abrahams V","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206422"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1569"},{"key":"e_1_2_6_22_2","first-page":"567","article-title":"Resistance of ovarian cancer cells to docetaxel is XIAP dependent and reversible by phenoxodiol","volume":"14","author":"Sapi E","year":"2004","journal-title":"Anticancer Drugs"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.12.030"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.191"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1093"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-05-088831"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2010.04190.x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509182102"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24317"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2008.191"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-3948"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1256"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006520"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0753-3322(94)90005-1"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003153"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-07-0118"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.1197"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1272"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-1244"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0897.2011.01068.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0897.2011.01068.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T11:16:42Z","timestamp":1698751002000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0897.2011.01068.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,14]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1111\/j.1600-0897.2011.01068.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0897.2011.01068.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,14]]}}}