{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T09:05:45Z","timestamp":1746090345826},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,10,5]],"date-time":"2011-10-05T00:00:00Z","timestamp":1317772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p><jats:bold>Citation<\/jats:bold> Rynekrova J, Kasparova D, Adamkova V, Fait T, Hubacek JA. Analysis of the potential role of apolipoprotein E polymorphism in genetic predisposition to spontaneous abortion. Am J Reprod Immunol 2012; 67: 179\u2013183<\/jats:p><jats:p><jats:bold>Problem\u2002<\/jats:bold> Up to 20% of pregnancies end in the first trimester by spontaneous abortion, but a significant number remains unexplained. The aim of this study is to investigate the role of variants within the gene for apolipoprotein E (<jats:italic>APOE<\/jats:italic>) in the genetic determination of spontaneous abortions.<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold> We collected DNA from 410 tissue samples of spontaneous abortions, and <jats:italic>APOE<\/jats:italic> was genotyped by PCR\u2013RFLP method. The frequencies were compared with a population sample of adults (<jats:italic>N<\/jats:italic>\u2003=\u20032606) and with a positive control (1060 women with at least two children).<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> The frequencies of the <jats:italic>APOE<\/jats:italic> genotypes in abortions (<jats:italic>APOE2E2 <\/jats:italic>+<jats:italic> E3E2 <\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.132; <jats:italic>APOE3E3\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.661; <jats:italic>APOE3E4\u2003<\/jats:italic>+<jats:italic>\u2003E4E4\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.195; <jats:italic>APOE4E2\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.012) did not significantly differ (<jats:italic>P<\/jats:italic>\u2003=\u20030.604) from the frequencies in analyzed adult population study (<jats:italic>APOE2E2\u2003<\/jats:italic>+<jats:italic>\u2003E3E2\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.132; <jats:italic>APOE3E3\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.686; <jats:italic>APOE3E4\u2003<\/jats:italic>+<jats:italic>\u2003E4E4\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.169; <jats:italic>APOE4E2\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.014) or from the positive control (<jats:italic>APOE2E2\u2003<\/jats:italic>+<jats:italic>\u2003E3E2\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.133; <jats:italic>APOE3E3\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.691; <jats:italic>APOE3E4\u2003<\/jats:italic>+<jats:italic> E4E4 <\/jats:italic>=0.166; <jats:italic>APOE4E2\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.010; <jats:italic>P<\/jats:italic>\u2003=\u20030.592).<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> Our study suggests that <jats:italic>APOE<\/jats:italic> may not be associated with spontaneous abortions in Caucasians.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2011.01071.x","type":"journal-article","created":{"date-parts":[[2011,10,5]],"date-time":"2011-10-05T17:00:25Z","timestamp":1317834025000},"page":"179-183","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of the Potential Role of Apolipoprotein E Polymorphism in Genetic Predisposition to Spontaneous Abortion"],"prefix":"10.1111","volume":"67","author":[{"given":"Jitka","family":"Rynekrova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dita","family":"Kasparova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Adamkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Fait","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslav 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