{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:50:36Z","timestamp":1771955436178,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,11,29]],"date-time":"2011-11-29T00:00:00Z","timestamp":1322524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p><jats:bold>Citation <\/jats:bold>Kim M\u2010G, Shim J\u2010Y, Pak JH, Jung B\u2010K, Won H\u2010S, Lee P\u2010R, Kim A. Progesterone modulates the expression of interleukin\u20106 in cultured term human uterine cervical fibroblasts. Am J Reprod Immunol 2012; 67: 369\u2013375<\/jats:p><jats:p><jats:bold>Problem\u2002<\/jats:bold> The preventative value of progesterone in preterm labor has been recently recognized, especially when it is administered via vaginal suppository. This study was undertaken to evaluate the effect of progesterone on interleukin\u20106 (IL\u20106) production in human uterine cervical fibroblasts (UCFs) treated with lipopolysaccharides (LPS).<\/jats:p><jats:p><jats:bold>Method of study\u2002<\/jats:bold> Human uterine cervical tissue was obtained at term, prior to the onset of labor, during the scheduled cesarean section or cesarean hysterectomy. Primary UCF cultures were established and confirmed by immunohistochemistry. IL\u20106 mRNA and protein expressions were examined by reverse\u2010transcription polymerase chain reaction and enzyme\u2010linked immunosorbent assay, respectively.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Lipopolysaccharides stimulation induced a clear time\u2010 and dose\u2010dependent increase in IL\u20106 mRNA and protein levels in UCFs (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Progesterone treatment significantly attenuated LPS\u2010induced increases in IL\u20106 mRNA and protein expressions in UCFs (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.05). Estrogen exposure had no effect on LPS\u2010induced IL\u20106 up\u2010regulation and did not modulate the effects of progesterone.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> Our preliminary results suggest that vaginal progesterone might prevent spontaneous preterm labor through a mechanism involving anti\u2010inflammatory effects on UCFs, particularly suppression of IL\u20106 production.<\/jats:p>","DOI":"10.1111\/j.1600-0897.2011.01094.x","type":"journal-article","created":{"date-parts":[[2011,11,30]],"date-time":"2011-11-30T13:34:16Z","timestamp":1322660056000},"page":"369-375","source":"Crossref","is-referenced-by-count":9,"title":["Progesterone Modulates the Expression of Interleukin\u20106 in Cultured Term Human Uterine Cervical Fibroblasts"],"prefix":"10.1111","volume":"67","author":[{"given":"Min\u2010Gyun","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae\u2010Yoon","family":"Shim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jhang Ho","family":"Pak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bok\u2010Kyung","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye\u2010Sung","family":"Won","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pil\u2010Ryang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahm","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(97)70432-0"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70347-0"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200005183422007"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2003.41"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa035140"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/14767050600949829"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.013698"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-0210"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.05.046"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1240001"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)91063-I"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.82.3.969"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-006-5194-z"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/0968051907080428"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20904"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/7.6.581"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2009.05.012"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2008.00633.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/09513590310001651777"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(97)00245-7"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0897.2011.01094.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0897.2011.01094.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T01:42:03Z","timestamp":1698716523000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0897.2011.01094.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,29]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1111\/j.1600-0897.2011.01094.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0897.2011.01094.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,29]]}}}