{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:02:39Z","timestamp":1790791359733,"version":"4.1.0"},"reference-count":72,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,4,26]],"date-time":"2012-04-26T00:00:00Z","timestamp":1335398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:sec><jats:title>Problem<\/jats:title><jats:p>Secretory leukocyte protease inhibitor (<jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content>) is an innate immune peptide present on the genitourinary tract mucosa that has antimicrobial activity. In this study, we investigated the interaction of <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> with <jats:italic>\n<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>eisseria gonorrhoeae<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>\n<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and far\u2010Western blots were used to analyze binding of <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> to gonococci. The binding site for <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> was identified by tryptic digests and mass spectrometry. Antimicrobial activity of <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> for gonococci was determined using bactericidal assays. <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> protein levels in cell supernatants were measured by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">SLPI mRNA<\/jats:styled-content> levels were assessed by quantitative <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SLPI bound directly to the gonococcal Opa protein and was bactericidal. Epithelial cells from the reproductive tract constitutively expressed <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> at different levels. Gonococcal infection of cells did not affect <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that <jats:styled-content style=\"fixed-case\">SLPI<\/jats:styled-content> is bactericidal for gonococci and is expressed by reproductive tract epithelial cells and thus is likely to play a role in the pathogenesis of gonococcal infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1600-0897.2012.01149.x","type":"journal-article","created":{"date-parts":[[2012,4,27]],"date-time":"2012-04-27T03:20:31Z","timestamp":1335496831000},"page":"116-127","source":"Crossref","is-referenced-by-count":29,"title":["Secretory Leukocyte Protease Inhibitor Binds to <i><scp>N<\/scp>eisseria gonorrhoeae<\/i> Outer Membrane Opacity Protein and is Bactericidal"],"prefix":"10.1111","volume":"68","author":[{"given":"Morris D.","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of 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