{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:44:44Z","timestamp":1770752684349,"version":"3.50.0"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,10,28]],"date-time":"2005-10-28T00:00:00Z","timestamp":1130457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dental Traumatology"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>Abstract\u2002\u2013\u2002<\/jats:bold> The use of alendronate, a bisphosphonate which is able to inhibit bone resorption, in order to prevent dental root resorption after tooth replantation would be of clinical relevance. However, this drug must be biocompatible to the periapical tissues. The aim of this study was to analyze the effect of an alendronate paste in polyethyleneglycol (2\u2003g\u2003ml<jats:sup>\u22121<\/jats:sup>) on endothelial cells in culture (<jats:italic>in vitro<\/jats:italic>) and on rat subcutaneous tissue (<jats:italic>in vivo<\/jats:italic>). For the <jats:italic>in vitro<\/jats:italic> study the paste was applied on round glass coverslips that were immersed into confluent cell cultures (clone Cips). The cell viability percentages of these cultures were obtained 0, 6 and 12\u2003h after contact with the substance. As control, cultures that received plain coverslips were used. This analysis was carried out in triplicate using the Trypan blue dye exclusion assay. For the <jats:italic>in vivo<\/jats:italic> study the paste was introduced into polyethylene tubes that were placed into the rat subcutaneous tissue. The rats were killed 7 and 14\u2003days later; then, the tissue sections stained with hematoxylin\u2013eosin were analyzed. <jats:italic>In vitro<\/jats:italic>, the alendronate caused a significant decrease in the cell viability in 6\u2003h (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) and 12\u2003h (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01), when compared with the control cultures. <jats:italic>In vivo<\/jats:italic> the tissue response was exuberant and similar at the two experimental times. There was a necrosis in a comprehensive area in contact with the open end of the tube. Presence of micro\u2010abscesses and intense inflammatory infiltrate in the hypoderm permeating the muscle fibers and fat lobules were observed. In conclusion, the alendronate paste in polyethylene glycol as used showed to be highly cytotoxic <jats:italic>in vitro<\/jats:italic> as well as <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1600-9657.2005.00370.x","type":"journal-article","created":{"date-parts":[[2005,10,28]],"date-time":"2005-10-28T15:22:43Z","timestamp":1130512963000},"page":"329-335","source":"Crossref","is-referenced-by-count":38,"title":["Cytotoxicity analysis of alendronate on cultured endothelial cells and subcutaneous tissue. 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