{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T04:28:48Z","timestamp":1697689728613},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,4,9]],"date-time":"2012-04-09T00:00:00Z","timestamp":1333929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dental Traumatology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:p><jats:bold>Abstract\u2002\u2013\u2002<\/jats:bold><jats:bold> Background:\u2002<\/jats:bold> Hank\u2019s balanced salt solution (HBSS) and milk have gained wide acceptance as storage media for avulsed tooth. However, the effect of the media and storage time on the periodontal ligament (PDL) cells involvement in the development of root resorption is still unclear. The purpose of this study was to evaluate whether precultured PDL cells in HBSS, milk, or modified Eagle\u2019s medium alpha (\u03b1\u2010MEM) would affect osteoclastogenesis.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> PDL cells were precultured in HBSS, milk, or \u03b1\u2010MEM for 1\u2003h or 6\u2003h before being co\u2010cultured with RAW 264.7 cells for an additional 3\u2003days for mRNA analysis and 11\u2003days for osteoclastogenesis assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Cyclooxygenase\u20102 (COX\u20102) mRNA was detected immediately in PDL cells precultured in the three storage media. The expression was up\u2010regulated markedly in all co\u2010cultures when compared with RAW cells alone. As a result of the co\u2010culture, interleukin\u20101\u03b2 (IL\u20101\u03b2) expression was detectable in both PDL and RAW cells. TRAP+ multinucleated, osteoclast\u2010like cells developed in all co\u2010cultures; the number of TRAP+ cells was highest (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) in the co\u2010cultures that PDL cells precultured in milk for 6\u2003h. The mRNA level of receptor activator of nuclear factor\u2010kappa B ligand (RANKL) was not detected in PDL cells. Osteoprotegerin (OPG) mRNA expression reduced with increased preculture time, but the difference was not significant (<jats:italic>P<\/jats:italic>\u2003&gt;\u20030.05).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> PDL cells kept in the three storage media led to TRAP+ multinucleated, osteoclast\u2010like cells formation via RANKL\u2010independent signaling. The ability to induce osteoclastogenesis may be considered as one of the factors to evaluate the ability of storage medium to maintain PDL viability after tooth avulsion.<\/jats:p>","DOI":"10.1111\/j.1600-9657.2012.01138.x","type":"journal-article","created":{"date-parts":[[2012,4,9]],"date-time":"2012-04-09T08:06:03Z","timestamp":1333958763000},"page":"59-65","source":"Crossref","is-referenced-by-count":14,"title":["Storage media enhance osteoclastogenic potential of human periodontal ligament cells via RANKL\u2010independent signaling"],"prefix":"10.1111","volume":"29","author":[{"given":"Xuan","family":"Zhan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengfei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waruna L.","family":"Dissanayaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary S. 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