{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:01:54Z","timestamp":1758121314837},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,8,31]],"date-time":"2006-08-31T00:00:00Z","timestamp":1156982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p><jats:bold>Objective:\u2002<\/jats:bold> To evaluate the effects of various 3\u2010hydroxy\u20103\u2010methylglutaryl\u2010coenzyme A reductase inhibitors (statins) on ectopic osteoinduction by recombinant human bone morphogenetic protein\u20102 (rhBMP\u20102) using different administration methods.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> Disks containing 5\u2003<jats:italic>\u03bc<\/jats:italic>g of rhBMP\u20102 and type I collagen were implanted into the calf muscles of 6\u2010week\u2010old male rats (<jats:italic>n<\/jats:italic>\u2003=\u200364). Either the lactone form of simvastatin (SV), open hydroxy\u2010acid form of simvastatin (SVA), cerivastatin (CVA), or vehicle (control) was then administered per orally (PO group) or subcutaneously (SC group) for 20\u2003days. The disks were removed on day 21 after implantation, and ectopic induced bone formation was evaluated by radiographic, histologic, and biochemical analysis.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Both the projected and radiopaque area on X\u2010ray film, and the calcium content of the SV group in the SC group (SV\u2013SC group) were significantly greater than those in the other SC and PO groups. Alkaline phosphatase activity and tartrate\u2010resistant acid phosphatase activity in the SV\u2013SC group were significantly lower than those in the other SC and PO groups. Histologic examination revealed an increase of ectopic induced bone volume in the SV\u2013SC group.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Subcutaneous administration of SV stimulates ectopic osteoinduction by rhBMP\u20102 through reduction of bone turnover.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2006.01271.x","type":"journal-article","created":{"date-parts":[[2006,8,31]],"date-time":"2006-08-31T15:43:31Z","timestamp":1157039011000},"page":"228-233","source":"Crossref","is-referenced-by-count":7,"title":["Subcutaneous administration of lactone form of simvastatin stimulates ectopic osteoinduction by rhBMP\u20102"],"prefix":"10.1111","volume":"13","author":[{"given":"T","family":"Sugiyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Mine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Murata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Tagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02400-4"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-2993(00)80107-4"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/atvb.21.10.1565"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02408-9"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.1.133"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1385\/JCD:5:2:151"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612013397762"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007740300001"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(97)01147-4"},{"issue":"1","key":"e_1_2_5_11_1","first-page":"S155","article-title":"Does statin use reduce risk of fracture in postmenopausal women? 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