{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T04:04:51Z","timestamp":1771560291699,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,6,7]],"date-time":"2007-06-07T00:00:00Z","timestamp":1181174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p><jats:bold>Objective:\u2002<\/jats:bold> Neutrophils play a crucial role in the defense of invading bacteria by releasing biologically active molecules. The response of peripheral blood neutrophils was studied in periodontitis\u2010affected patients and in healthy controls towards stimulation to <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>Pg<\/jats:italic>) and <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> (<jats:italic>Aa<\/jats:italic>) extracts.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> Peripheral venous blood was drawn from 23 adult patients with moderate to advanced chronic periodontitis (probing depth \u22655\u2003mm, attachment loss \u22653\u2003mm), and 30 healthy volunteers. Neutrophil response followed by metalloproteinase\u20109 (MMP\u20109) and interleukin\u20108 (IL\u20108) secretion was assayed by zymography and enzyme\u2010linked immunosorbent assay, respectively, on both whole blood and purified neutrophils. In addition to periodontal pathogen extracts, known stimulating agents were tested, such as <jats:italic>Escherichia coli<\/jats:italic>\u2010lipopolysaccharide (LPS), phytohemagglutinin, and zymosan A.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Neutrophil response, expressed as a secretion ratio under stimulated and non\u2010stimulated conditions, measured in whole blood, showed no differences between periodontitis and healthy controls. Instead, in purified neutrophils from patients, MMP\u20109 exhibited a significantly higher secretion ratio with LPS and <jats:italic>Pg<\/jats:italic> (1.5\u2010 to 2\u2010fold), whereas IL\u20108 showed a larger increase in secretion ratio (3\u2010 to 7\u2010fold) in the presence of <jats:italic>Pg<\/jats:italic>, <jats:italic>Aa<\/jats:italic>, LPS, and zymosan A.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Peripheral neutrophils of periodontitis\u2010affected patients are more reactive as suggested by their significantly higher response toward periodontal pathogen extracts and other stimulating agents.<\/jats:p>","DOI":"10.1111\/j.1601-0825.2006.01323.x","type":"journal-article","created":{"date-parts":[[2007,6,7]],"date-time":"2007-06-07T13:26:48Z","timestamp":1181222808000},"page":"474-481","source":"Crossref","is-referenced-by-count":16,"title":["Stimulatory response of neutrophils from periodontitis patients with periodontal pathogens"],"prefix":"10.1111","volume":"13","author":[{"given":"CG","family":"Resta\u00edno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Chaparro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MA","family":"Valenzuela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AM","family":"Kettlun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Vernal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Puente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MP","family":"Jaque","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Gamonal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(02)00013-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-8-reviews3011"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.1.403"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1128\/iai.24.1.233-243.1979","article-title":"Interaction of inflammatory cells and oral microorganisms. 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