{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:04:58Z","timestamp":1787015098132,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,12,18]],"date-time":"2007-12-18T00:00:00Z","timestamp":1197936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:\u2002<\/jats:bold>\n                    Current methods for determining salivary antibodies are cumbersome for large\u2010scale screenings.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Objectives:\u2002<\/jats:bold>\n                    To test checkerboard immunodetection for monitoring salivary antibodies and to profile them in diabetic individuals with periodontitis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:\u2002<\/jats:bold>\n                    Salivary anti\u2010\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    , anti\u2010\n                    <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic>\n                    and total IgA levels of 10 individuals were compared using checkerboard immunoblotting and enzyme\u2010linked immunosorbent assay (ELISA).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:\u2002<\/jats:bold>\n                    Close correlation between both methods was found in anti\u2010\n                    <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                    IgA and total IgA, but not in anti\u2010\n                    <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>\n                    IgA, because of high background levels in ELISA. Thereafter, checkerboard immunodetection was used to compare salivary antibodies of 20 adult type II diabetic with 32 non\u2010diabetic individuals with (\n                    <jats:italic>n<\/jats:italic>\n                    \u2003=\u200322) or without (\n                    <jats:italic>n<\/jats:italic>\n                    \u2003=\u200310) periodontitis. Patients with periodontitis (regardless of their diabetic condition) expressed increased levels of total IgA in both whole and parotid saliva, but reduced levels of anti\u2010\n                    <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>\n                    IgA in whole saliva. Consequently, the proportion of anti\u2010\n                    <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>\n                    IgA in the total IgA was lower in saliva of patients with periodontitis compared with healthy controls.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:\u2002<\/jats:bold>\n                    Checkerboard immunodetection was reliable and economical for screening saliva samples for multiple antibody reactions. Our results support previous reports which suggested that patients with periodontitis are able to secrete high levels of salivary Ig, but are hampered in targeting their salivary response toward\n                    <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/j.1601-0825.2006.01345.x","type":"journal-article","created":{"date-parts":[[2010,8,6]],"date-time":"2010-08-06T10:20:49Z","timestamp":1281090049000},"page":"51-59","source":"Crossref","is-referenced-by-count":18,"title":["Assessing the levels of immunoglobulins in the saliva of diabetic individuals with periodontitis using checkerboard 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